Does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats?
Does dietary fibre stimulate intestinal epithelial cell proliferation in germ free rats?
复制标题
膳食纤维是否会刺激无菌大鼠肠上皮细胞增殖？
作者：
R. Goodlad;B. Ratcliffe;J. Fordham;N. Wright
The aim of the present experiment was to investigate the role of hind gut fermentation in the proliferative response of the intestinal epithelium to dietary fibre. We have previously shown that refeeding starved rats with an elemental diet supplemented with fermentable dietary fibre (but not inert bulk) is capable of stimulating intestinal epithelial cell proliferation throughout the gastrointestinal tract. Three groups of 10 germ free (GF) rats and three groups of 10 conventional (CV) rats, were used. All groups were starved for three days and then refed for two days with either an elemental diet (Flexical); Flexical plus 30% kaolin; or Flexical plus 30% of a fibre mixture. Cell production was determined by the accumulation of vincristine arrested metaphases in microdissected crypts. There was no significant difference between refeeding the rats with an elemental diet alone or with kaolin supplementation, however, the addition of fibre in CV rats was associated with a significant increase in intestinal crypt cell production rate in both the small intestine (p less than 0.01) and the colon (p less than 0.001). This marked proliferative effects of fibre was abolished in the GF rats. It can be concluded that it is the products of hind gut fermentation, not fibre per se that stimulate intestinal epithelial cell proliferation in the colon and small intestine.
喂食高纤维饮食的 1,2-二甲基肼治疗大鼠的结肠腔 pH 值、细胞增殖和结肠癌发生之间的关系。
影响因子：
11.2
作者：
Jacobs,LR;Lupton,JR
通讯作者：
Lupton,JR
膳食玉米麸和自水解木质素对 3,2-二甲基-4-氨基联苯诱导的雄性 F344 大鼠肠道癌发生的影响。
DOI：
--
发表时间：
1983
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Reddy,BS;Maeura,Y;Wayman,M
通讯作者：
Wayman,M
膳食纤维对结肠细胞增殖的影响及其与结肠癌发生的关系。
影响因子：
5.1
作者：
Jacobs,LR
通讯作者：
Jacobs,LR