The Intrapopulation Genetic Diversity of RNA Virus May Influence the Sensitivity of Chlorine Disinfection.
The Intrapopulation Genetic Diversity of RNA Virus May Influence the Sensitivity of Chlorine Disinfection.
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DOI：
10.3389/fmicb.2022.839513
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发表时间：
2022
影响因子：
5.2
通讯作者：
Sano, Daisuke
中科院分区：
文献类型：
--
作者：
Kadoya, Syun-suke;Urayama, Syun-ichi;Nunoura, Takuro;Hirai, Miho;Takaki, Yoshihiro;Kitajima, Masaaki;Nakagomi, Toyoko;Nakagomi, Osamu;Okabe, Satoshi;Nishimura, Osamu;Sano, Daisuke
RNA病毒群体不是克隆的;相反，它们包含一个突变群，其中序列与主序列略有不同。种群内的遗传多样性（种群内遗传多样性）对于RNA病毒在环境压力下生存至关重要。消毒已成为一个重要的做法，在控制致病性病毒，然而，种群内的遗传多样性对消毒的敏感性，定义为-log 10（消毒后感染滴度/消毒前滴度）的影响，尚未阐明。在这项研究中，我们连续传代恒河猴轮状病毒的群体。我们证明了氯敏感性降低的人群是随机出现的，与氯暴露无关。测序分析表明，与敏感群体相比，低敏感群体具有较高的外衣壳蛋白基因的非同义遗传多样性，表明外衣壳蛋白氨基酸序列的变化是影响氯敏感性的主要因素。在不太敏感的人群中没有发现常见的突变，这表明，而不是特定的突变，外部衣壳蛋白本身的多样性与消毒敏感性和消毒敏感性随机变化。模拟结果表明，消毒敏感性的遗传多样性的人口是不稳定的，如果合作的病毒簇，包括多个序列的形成。这些结果表明，任何导致低种群内遗传多样性的预防措施对于防止致病性RNA病毒在社会中的传播和进化都很重要。
RNA virus populations are not clonal; rather, they comprise a mutant swarm in which sequences are slightly different from the master sequence. Genetic diversity within a population (intrapopulation genetic diversity) is critical for RNA viruses to survive under environmental stresses. Disinfection has become an important practice in the control of pathogenic viruses; however, the impact of intrapopulation genetic diversity on the sensitivity of disinfection, defined as –log10 (postdisinfected infectious titer/predisinfected titer), has not been elucidated. In this study, we serially passaged populations of rhesus rotavirus. We demonstrated that populations with reduced chlorine sensitivity emerged at random and independently of chlorine exposure. Sequencing analysis revealed that compared with sensitive populations, less-sensitive ones had higher non-synonymous genetic diversity of the outer capsid protein gene, suggesting that changes in the amino acid sequences of the outer capsid protein were the main factors influencing chlorine sensitivity. No common mutations were found among less-sensitive populations, indicating that rather than specific mutations, the diversity of the outer capsid protein itself was associated with the disinfection sensitivity and that the disinfection sensitivity changed stochastically. Simulation results suggest that the disinfection sensitivity of a genetically diverse population is destabilized if cooperative viral clusters including multiple sequences are formed. These results advocate that any prevention measures leading to low intrapopulation genetic diversity are important to prevent the spread and evolution of pathogenic RNA viruses in society.
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影响因子：
6.7
作者：
Bordería AV;Isakov O;Moratorio G;Henningsson R;Agüera-González S;Organtini L;Gnädig NF;Blanc H;Alcover A;Hafenstein S;Fontes M;Shomron N;Vignuzzi M
通讯作者：
Vignuzzi M
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1016/j.jmoldx.2017.01.011
发表时间：
2017-05
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
--
作者：
Jennings LJ;Arcila ME;Corless C;Kamel-Reid S;Lubin IM;Pfeifer J;Temple-Smolkin RL;Voelkerding KV;Nikiforova MN
通讯作者：
Nikiforova MN
影响因子：
3.8
作者：
Ciota, Alexander T.;Ngo, Kiet A.;Kramer, Laura D.
通讯作者：
Kramer, Laura D.
影响因子：
3.7
作者：
Lema, Cristina;Torres, Carolina;Gomez, Ricardo M.
通讯作者：
Gomez, Ricardo M.