Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles.
Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles.
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β-微管蛋白糖基化结构域对微管细胞器组装和大小的细胞环境特异性影响。
DOI：
10.1091/mbc.e04-03-0247
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发表时间：
2004
影响因子：
3.3
通讯作者：
Gaertig,Jacek
中科院分区：
文献类型：
--
作者：
Thazhath,Rupal;Jerka-Dziadosz,Maria;Duan,Jianming;Wloga,Dorota;Gorovsky,MartinA;Frankel,Joseph;Gaertig,Jacek
Tubulin glycylation is a posttranslational modification found in cells with cilia or flagella. The ciliateTetrahymenahas glycylation on ciliary and cortical microtubules. We showed previously that mutating three glycylation sites on β-tubulin produces immotile 9 + 0 axonemes and inhibits cytokinesis. Here, we use an inducible glycylation domain mutation and epitope tagging to evaluate the potential of glycylation-deficient tubulin for assembly and maintenance of microtubular systems. In axonemes, the major defects, including lack of the central pair, occurred during assembly, and newly made cilia were abnormally short. The glycylation domain also was required for maintenance of the length of already assembled cilia. In contrast to the aberrant assembly of cilia, several types of cortical organelles showed an abnormally high number of microtubules in the same mutant cells. Thus, the consequences of deficiency in tubulin glycylation are organelle type specific and lead to either insufficient assembly (cilia) or excessive assembly (basal bodies and cortical microtubules). We suggest that the diverse functions of the β-tubulin glycylation domain are executed by spatially restricted microtubule-associated proteins.
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非生长四膜虫纤毛再生过程中微管蛋白的调节。
影响因子：
3.7
作者：
E. Nelsen
通讯作者：
E. Nelsen
通过串联质谱法对微管蛋白翻译后多糖基化进行结构表征。
影响因子：
2.9
作者：
Joëlle Vinh;James I. Langridge;M. Bré;N. Levilliers;V. Redeker;Denis Loyaux;Jean Rossier
通讯作者：
Jean Rossier
梨形四膜虫纤毛的中央微管终止。
DOI：
10.1016/0309-1651(77)90008-x
发表时间：
1977
期刊：
Cell biology international reports
影响因子：
--
作者：
W. Sale;P. Satir
通讯作者：
P. Satir
影响因子：
3.9
作者：
ADOUTTE, A;CLAISSE, M;BEISSON, J
通讯作者：
BEISSON, J
影响因子：
3.3
作者：
Bré, MH;Redeker, V;Levilliers, N
通讯作者：
Levilliers, N