The Wheat Bax Inhibitor-1 Protein Interacts with an Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis.
The Wheat Bax Inhibitor-1 Protein Interacts with an Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis.
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小麦 Bax Inhibitor-1 蛋白与水通道蛋白 TaPIP1 相互作用并增强拟南芥的抗病性
DOI：
10.3389/fpls.2018.00020
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发表时间：
2018
影响因子：
5.6
通讯作者：
Xu ZS
中科院分区：
文献类型：
--
作者：
Lu PP;Yu TF;Zheng WJ;Chen M;Zhou YB;Chen J;Ma YZ;Xi YJ;Xu ZS
Bax抑制剂-1（BI-1）是一种在真核生物中进化上保守的内质网（ER）驻留的细胞死亡抑制因子。BI-1在拟南芥中抑制生物和非生物胁迫的能力已得到充分研究，而小麦BI-1的功能在很大程度上是未知的。在这项研究中，小麦BI-1基因TaBI-1.1是通过RNA-seq分析禾谷镰刀菌（Fg）处理的小麦分离。TaBI-1.1表达由水杨酸（SA）处理诱导，并由脱落酸（ABA）处理下调。基于β-葡萄糖醛酸酶（GUS）染色，TaBI-1.1在成熟叶和根中表达，而在下胚轴或幼叶中不表达。TaBI-1.1基因在拟南芥中的组成型表达增强了拟南芥对假单胞菌的抗性。番茄（Pst）DC3000感染并诱导SA相关基因表达。此外，TaBI-1.1转基因拟南芥表现出减轻由高浓度SA引起的损伤，并降低ABA的敏感性。与表型一致，35S：：TaBI-1.1和Col-0植物的RNA-seq分析显示TaBI-1.1参与生物胁迫。这些结果表明TaBI-1.1正调控SA信号，在植物对生物胁迫的响应中起重要作用。此外，TaBI-1.1与水通道蛋白TaPIP1相互作用，二者均定位于内质网膜。此外，我们证明了TaPIP1被SA处理上调，TaPIP1转基因拟南芥增强了对Pst DC3000感染的抗性。因此，内质网膜上的TaBI-1.1和TaPIP1之间的相互作用可能是响应SA信号和防御反应而发生的。
Bax inhibitor-1 (BI-1) is an endoplasmic reticulum (ER)-resident cell death suppressor evolutionarily conserved in eukaryotes. The ability of BI-1 to inhibit the biotic and abiotic stresses have been well-studied in Arabidopsis, while the functions of wheat BI-1 are largely unknown. In this study, the wheat BI-1 gene TaBI-1.1 was isolated by an RNA-seq analysis of Fusarium graminearum (Fg)-treated wheat. TaBI-1.1 expression was induced by a salicylic acid (SA) treatment and down-regulated by an abscisic acid (ABA) treatment. Based on β-glucuronidase (GUS) staining, TaBI-1.1 was expressed in mature leaves and roots but not in the hypocotyl or young leaves. Constitutive expression of TaBI-1.1 in Arabidopsis enhanced its resistance to Pseudomonas syringae pv. Tomato (Pst) DC3000 infection and induced SA-related gene expression. Additionally, TaBI-1.1 transgenic Arabidopsis exhibited an alleviation of damage caused by high concentrations of SA and decreased the sensitivity to ABA. Consistent with the phenotype, the RNA-seq analysis of 35S::TaBI-1.1 and Col-0 plants showed that TaBI-1.1 was involved in biotic stresses. These results suggested that TaBI-1.1 positively regulates SA signals and plays important roles in the response to biotic stresses. In addition, TaBI-1.1 interacted with the aquaporin TaPIP1, and both them were localized to ER membrane. Furthermore, we demonstrated that TaPIP1 was up-regulated by SA treatment and TaPIP1 transgenic Arabidopsis enhanced the resistance to Pst DC3000 infection. Thus, the interaction between TaBI-1.1 and TaPIP1 on the ER membrane probably occurs in response to SA signals and defense response.
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影响因子：
5.1
作者：
Aubert, D;Chevillard, M;Herzog, M
通讯作者：
Herzog, M
影响因子：
9.4
作者：
Choi, Changhyun;Hwang, Seon-Hee;Hwang, Duk-Ju
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Hwang, Duk-Ju
影响因子：
7.4
作者：
Garretón, V;Carpinelli, J;Holuigue, L
通讯作者：
Holuigue, L
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Chae, HJ;Ke, N;Reed, JC
通讯作者：
Reed, JC