Metabolome variations in the Porphyromonas gingivalis vimA mutant during hydrogen peroxide-induced oxidative stress.
Metabolome variations in the Porphyromonas gingivalis vimA mutant during hydrogen peroxide-induced oxidative stress.
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DOI：
10.1111/omi.12075
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发表时间：
2015-04
影响因子：
3.7
通讯作者：
Fletcher HM
中科院分区：
文献类型：
--
作者：
McKenzie RM;Aruni W;Johnson NA;Robles A;Dou Y;Henry L;Boskovic DS;Fletcher HM
牙龈卟啉单胞菌在牙周袋的氧化微环境中的适应性和存活率是其生存和毒力不可缺少的，并受到多种系统的调节。在参与牙龈假单胞菌氧化应激抗性的各种基因中，VIMA基因是6.15kb基因座的一部分。为了阐明牙龈假单胞菌VimA缺陷突变体在氧化应激抵抗中的作用，我们使用了一种全球方法来评估转录谱，研究在典型的牙周袋环境中影响生存和毒力的独特代谢组变化。在牙龈假单胞菌FLL92中发现了一种针对氧化应激的多层保护策略，并上调了解毒基因。氧化应激的持续时间对转录有不同的调节作用，其中94个(87%)基因在10min内上调一倍，55个(83.3%)基因在15min内上调。大多数上调的基因(55%)属于假设/未知/未分配的功能类别。代谢组的变化表现为富马酸和甲醛的减少，因此求助于替代能源的产生和维持减少的代谢状态。金属离子结合蛋白运输和分泌系统的编码基因转座酶上调。
The adaptability and survival of Porphyromonas gingivalis in the oxidative microenvironment of the periodontal pocket are indispensable for survival and virulence, and are modulated by multiple systems. Among the various genes involved in P. gingivalis oxidative stress resistance, vimA gene is a part of the 6.15-kb locus. To elucidate the role of a P. gingivalis vimA-defective mutant in oxidative stress resistance, we used a global approach to assess the transcriptional profile, to study the unique metabolome variations affecting survival and virulence in an environment typical of the periodontal pocket. A multilayered protection strategy against oxidative stress was noted in P. gingivalis FLL92 with upregulation of detoxifying genes. The duration of oxidative stress was shown to differentially modulate transcription with 94 (87%) genes upregulated twofold during 10 min and 55 (83.3%) in 15 min. Most of the up-regulated genes (55%), fell in the hypothetical/unknown/unassigned functional class. Metabolome variation showed reduction in fumarate and formaldehyde, hence resorting to alternative energy generation and maintenance of a reduced metabolic state. There was upregulation of transposases, genes encoding for the metal ion binding protein transport and secretion system.
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影响因子：
3.2
作者：
Henry, Leroy G.;Sandberg, Lawrence;Fletcher, Hansel M.
通讯作者：
Fletcher, Hansel M.
DOI：
10.1038/nrmicro1949
发表时间：
2009-02
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.1
作者：
Abaibou, H;Chen, Z;Fletcher, HM
通讯作者：
Fletcher, HM
影响因子：
3.1
作者：
Aruni, A. Wilson;Lee, J.;Fletcher, H. M.
通讯作者：
Fletcher, H. M.
影响因子：
3.1
作者：
Henry LG;McKenzie RM;Robles A;Fletcher HM
通讯作者：
Fletcher HM