Identification of a two-marker-haplotype on Bos taurus autosome 18 associated with somatic cell score in German Holstein cattle.
Identification of a two-marker-haplotype on Bos taurus autosome 18 associated with somatic cell score in German Holstein cattle.
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DOI：
10.1186/1471-2156-10-50
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发表时间：
2009-09-02
期刊：
影响因子：
2.9
通讯作者：
Kühn C
中科院分区：
文献类型：
--
作者：
Brand B;Baes C;Mayer M;Reinsch N;Kühn C
在许多国家，体细胞评分（SCS）作为乳房健康的指标性状在常规公畜评价中实施。体细胞评分与临床型乳腺炎高度相关，在德国荷斯坦牛群体中，SCS的数量性状基因座（QTL）已被反复定位在普通牛常染色体18（BTA 18）上。在本研究中，我们报告了一个精细的分析先前检测到的QTL区域BTA 18的目的是确定标记和标记单倍型与SCS连锁不平衡。一个联合的连锁和连锁不平衡的方法进行了实施，并进行关联分析的标记基因型和母系遗传的两个标记单倍型，以确定标记和单倍型的连锁不平衡与一个位点影响SCS在德国荷斯坦人口。我们在BTA 18上的中间至端粒区域的标记区间9内检测到全基因组显著QTL（HAMP_c.366+ 109 G> A-BMS 833），并且在标记区间12-13内检测到第二个推定QTL（BB 710- PVRL2_c.392G>A）。关联分析与基因型的标记侧翼最有可能的QTL的位置揭示了微卫星标记BMS 833（间隔9）与一个位点影响SCS的家庭内调查。对母系遗传的两个标记单倍型和母系遗传的两个标记间隔配子的影响的进一步分析表明，标记间隔12-13中的单倍型249-G（BB 710- PVRL2_c.392G>A）与德国荷斯坦牛群体的SCS相关。我们的研究结果证实了以前的QTL定位结果，SCS和支持的假设，一个以上的基因座可能会影响乳房健康的中部端粒区的BTA 18。然而，需要对所报道的QTL区域进行后续调查以验证双QTL假设并确认标记区间12-13中的双标记单倍型249-G（BB 710- PVRL2_c.392G>A）与SCS的关联。为此，需要更高的标记密度和多性状和多QTL模型来缩小德国荷斯坦牛中影响SCS的因果突变或突变的位置。
The somatic cell score (SCS) is implemented in routine sire evaluations in many countries as an indicator trait for udder health. Somatic cell score is highly correlated with clinical mastitis, and in the German Holstein population quantitative trait loci (QTL) for SCS have been repeatedly mapped on Bos taurus autosome 18 (BTA18). In the present study, we report a refined analysis of previously detected QTL regions on BTA18 with the aim of identifying marker and marker haplotypes in linkage disequilibrium with SCS. A combined linkage and linkage disequilibrium approach was implemented, and association analyses of marker genotypes and maternally inherited two-marker-haplotypes were conducted to identify marker and haplotypes in linkage disequilibrium with a locus affecting SCS in the German Holstein population. We detected a genome-wide significant QTL within marker interval 9 (HAMP_c.366+109G>A - BMS833) in the middle to telomeric region on BTA18 and a second putative QTL in marker interval 12-13 (BB710 - PVRL2_c.392G>A). Association analyses with genotypes of markers flanking the most likely QTL positions revealed the microsatellite marker BMS833 (interval 9) to be associated with a locus affecting SCS within the families investigated. A further analysis of maternally inherited two-marker haplotypes and effects of maternally inherited two-marker-interval gametes indicated haplotype 249-G in marker interval 12-13 (BB710 - PVRL2_c.392G>A) to be associated with SCS in the German Holstein population. Our results confirmed previous QTL mapping results for SCS and support the hypothesis that more than one locus presumably affects udder health in the middle to telomeric region of BTA18. However, a subsequent investigation of the reported QTL regions is necessary to verify the two-QTL hypothesis and confirm the association of two-marker-haplotype 249-G in marker interval 12-13 (BB710 - PVRL2_c.392G>A) with SCS. For this purpose, higher marker density and multiple-trait and multiple-QTL models are required to narrow down the position of the causal mutation or mutations affecting SCS in German Holstein cattle.
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