Exportin-5 orthologues are functionally divergent among species.
Exportin-5 orthologues are functionally divergent among species.
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DOI：
10.1093/nar/gkl663
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发表时间：
2006
影响因子：
14.9
通讯作者：
Yoneda Y
中科院分区：
文献类型：
--
作者：
Shibata S;Sasaki M;Miki T;Shimamoto A;Furuichi Y;Katahira J;Yoneda Y
Exportin-5是一种进化上保守的核输出因子，属于importin-β蛋白家族，已知其在哺乳动物细胞中非编码小RNA（如microRNA、病毒微螺旋RNA和tRNA的子集的前体）的核输出中发挥作用。在这项研究中，我们发现，来自不同物种，如人类，果蝇和酵母的exportin-5的直系同源物表现出不同的功能。我们发现，Msn 5 p，酵母exportin-5的直系同源物，结合双链RNA，它更喜欢一个较短的22 nt，双链RNA到1080 nt的pre-miRNA，即使这两个RNA共享一个类似的末端结构。此外，我们发现果蝇exportin-5与前体miRNA结合，并且在测试的exportin-5直系同源物中，它对tRNA表现出最高的亲和力。在培养细胞中敲低果蝇exportin-5降低了tRNA和miRNA的量，而在培养细胞中敲低人exportin-5只影响miRNA而不影响tRNA水平。这些结果表明，双链RNA结合能力是exportin-5直向同源物的遗传功能特征，并且果蝇exportin-5在缺乏用于exportin-t的直向同源基因的果蝇中作为tRNA以及前体miRNA的输出者发挥功能。
Exportin-5, an evolutionarily conserved nuclear export factor belonging to the importin-β family of proteins, is known to play a role in the nuclear export of small noncoding RNAs such as precursors of microRNA, viral minihelix RNA and a subset of tRNAs in mammalian cells. In this study, we show that the exportin-5 orthologues from different species such as human, fruit fly and yeast exhibit diverged functions. We found that Msn5p, a yeast exportin-5 orthologue, binds double-stranded RNAs and that it prefers a shorter 22 nt, double-stranded RNA to ∼80 nt pre-miRNA, even though both of these RNAs share a similar terminal structure. Furthermore, we found that Drosophila exportin-5 binds pre-miRNAs and that amongst the exportin-5 orthologues tested, it shows the highest affinity for tRNAs. The knockdown of Drosophila exportin-5 in cultured cells decreased the amounts of tRNA as well as miRNA, whereas the knock down of human exportin-5 in cultured cells affected only miRNA but not tRNA levels. These results indicate that double-stranded RNA binding ability is an inherited functional characteristic of the exportin-5 orthologues and that Drosophila exportin-5 functions as an exporter of tRNAs as well as pre-miRNAs in the fruit fly that lacks the orthologous gene for exportin-t.
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DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE
影响因子：
4.5
作者：
Lipowsky, G;Bischoff, FR;Görlich, D
通讯作者：
Görlich, D
影响因子：
16
作者：
Kutay, U;Lipowsky, G;Gorlich, D
通讯作者：
Gorlich, D
影响因子：
11.4
作者：
Bohnsack, MT;Regener, K;Görlich, D
通讯作者：
Görlich, D
影响因子：
11.4
作者：
Arts, GJ;Kuersten, S;Mattaj, IW
通讯作者：
Mattaj, IW