Quantification of Microbial Communities in Subsurface Marine Sediments of the Black Sea and off Namibia.
Quantification of Microbial Communities in Subsurface Marine Sediments of the Black Sea and off Namibia.
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DOI：
10.3389/fmicb.2012.00016
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发表时间：
2012
影响因子：
5.2
通讯作者：
Siegert M
中科院分区：
文献类型：
--
作者：
Schippers A;Kock D;Höft C;Köweker G;Siegert M
在流星 72-5 和 76-1 号科考巡航期间，分别在缺氧黑海和纳米比亚附近本格拉上升流系统的六个站点，通过重力取芯，在海底以下 10 米的深度取了富含有机物的地下海洋沉积物。使用总细胞计数、催化报告基因沉积-荧光原位杂交 (CARD-FISH) 和定量实时 PCR (Q-PCR) 分析不同沉积物深度的定量微生物群落组成。总细胞计数随着深度的增加而减少，从沉积物表面的 109 至 1010 个细胞/mL 到一米深度以下的 107-109 个细胞/mL。根据 CARD-FISH 和 Q-PCR 分析，发现细菌和古细菌的比例总体相似。使用 Q-PCR 定量原核和真核小亚基核糖体 RNA 基因（16S 和 18S rRNA）以及参与不同生物地球化学过程的功能基因的核心分布。 Crenarchaeota 和候选细菌门 JS-1 以及 Chloroflexi 门的 Anaerolineae 类和 Caldilineae 类的含量非常丰富。真核生物以及还原金属和硫酸盐的地杆菌科（仅在受本格拉上升流影响的沉积物中）含量较少，但在大多数样品中均可检测到。在本格拉上升流和黑海沉积物中检测到了编码 RuBisCO 大亚基的功能基因 cbbL、指示硫酸盐还原剂的基因 dsrA 和 aprA 以及产甲烷菌的 mcrA 基因。总体而言，沉积物的高有机碳含量伴随着高细胞计数和高基因拷贝数，以及同等丰度的细菌和古细菌。
Organic-rich subsurface marine sediments were taken by gravity coring up to a depth of 10 m below seafloor at six stations from the anoxic Black Sea and the Benguela upwelling system off Namibia during the research cruises Meteor 72-5 and 76-1, respectively. The quantitative microbial community composition at various sediment depths was analyzed using total cell counting, catalyzed reporter deposition – fluorescence in situ hybridization (CARD–FISH) and quantitative real-time PCR (Q-PCR). Total cell counts decreased with depths from 109 to 1010 cells/mL at the sediment surface to 107–109 cells/mL below one meter depth. Based on CARD–FISH and Q-PCR analyses overall similar proportions of Bacteria and Archaea were found. The down-core distribution of prokaryotic and eukaryotic small subunit ribosomal RNA genes (16S and 18S rRNA) as well as functional genes involved in different biogeochemical processes was quantified using Q-PCR. Crenarchaeota and the bacterial candidate division JS-1 as well as the classes Anaerolineae and Caldilineae of the phylum Chloroflexi were highly abundant. Less abundant but detectable in most of the samples were Eukarya as well as the metal and sulfate-reducing Geobacteraceae (only in the Benguela upwelling influenced sediments). The functional genes cbbL, encoding for the large subunit of RuBisCO, the genes dsrA and aprA, indicative of sulfate-reducers as well as the mcrA gene of methanogens were detected in the Benguela upwelling and Black Sea sediments. Overall, the high organic carbon content of the sediments goes along with high cell counts and high gene copy numbers, as well as an equal abundance of Bacteria and Archaea.
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影响因子：
4.2
作者：
Coolen, Marco J. L.;Shtereva, Galina
通讯作者：
Shtereva, Galina
影响因子：
--
作者：
Coolen, MJL;Boere, A;Damsté, JSS
通讯作者：
Damsté, JSS
影响因子：
4.4
作者：
Holmes, DE;Finneran, KT;Lovley, DR
通讯作者：
Lovley, DR
DOI：
10.1073/pnas.0600035103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Biddle, JF;Lipp, JS;Hinrichs, KU
通讯作者：
Hinrichs, KU
影响因子：
56.9
作者：
D'Hondt, S;Jorgensen, BB;Acosta, JLS
通讯作者：
Acosta, JLS