Distinct cell clusters touching islet cells induce islet cell replication in association with over-expression of Regenerating Gene (REG) protein in fulminant type 1 diabetes.
Distinct cell clusters touching islet cells induce islet cell replication in association with over-expression of Regenerating Gene (REG) protein in fulminant type 1 diabetes.
复制标题
DOI：
10.1371/journal.pone.0095110
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kobayashi T
中科院分区：
文献类型：
--
作者：
Aida K;Saitoh S;Nishida Y;Yokota S;Ohno S;Mao X;Akiyama D;Tanaka S;Awata T;Shimada A;Oikawa Y;Shimura H;Furuya F;Takizawa S;Ichijo M;Ichijo S;Itakura J;Fujii H;Hashiguchi A;Takasawa S;Endo T;Kobayashi T
胰岛内分泌细胞的支持结构，包括胰岛包裹基底膜(BMS)、细胞外基质(ECM)和可能的细胞团，目前尚不清楚。在非糖尿病状态和人类暴发性1型糖尿病的炎症环境中，研究了胰岛细胞团周围的结构，包括骨髓基质、细胞外基质和胰腺腺泡样细胞团。免疫组织化学和电子显微镜分析表明，人胰岛细胞团和腺泡样细胞团直接粘连在一起，两者之间有桥粒结构和包被的坑状结构。这两个细胞团被包裹在由普通的BMS/ECM组成的连续的包膜中。腺泡样细胞团含有含有再生(REG)Iα蛋白的小泡。含有Reg Iα蛋白的囊泡直接分泌到胰岛细胞。在暴发型1型糖尿病的炎症环境中，腺泡样细胞团过度表达REG Iα蛋白。胰岛内分泌细胞，包括β细胞和非β细胞，被腺泡样细胞团包裹，表现出自我复制，Ki67阳性细胞数量明显增加。与胰岛内分泌细胞接触的腺泡样细胞团明显，因为细胞团被胰岛簇团包裹，并被普通的BMS/ECM包围。此外，腺泡样细胞团表达Reg Iα蛋白，并直接分泌到邻近的非糖尿病状态的胰岛内分泌细胞；在暴发型1型糖尿病炎症环境中，腺泡样细胞团过度表达Reg Iα，胰岛细胞明显自我复制。
Pancreatic islet endocrine cell-supporting architectures, including islet encapsulating basement membranes (BMs), extracellular matrix (ECM), and possible cell clusters, are unclear. The architectures around islet cell clusters, including BMs, ECM, and pancreatic acinar-like cell clusters, were studied in the non-diabetic state and in the inflamed milieu of fulminant type 1 diabetes in humans. Immunohistochemical and electron microscopy analyses demonstrated that human islet cell clusters and acinar-like cell clusters adhere directly to each other with desmosomal structures and coated-pit-like structures between the two cell clusters. The two cell-clusters are encapsulated by a continuous capsule composed of common BMs/ECM. The acinar-like cell clusters have vesicles containing regenerating (REG) Iα protein. The vesicles containing REG Iα protein are directly secreted to islet cells. In the inflamed milieu of fulminant type 1 diabetes, the acinar-like cell clusters over-expressed REG Iα protein. Islet endocrine cells, including beta-cells and non-beta cells, which were packed with the acinar-like cell clusters, show self-replication with a markedly increased number of Ki67-positive cells. The acinar-like cell clusters touching islet endocrine cells are distinct, because the cell clusters are packed with pancreatic islet clusters and surrounded by common BMs/ECM. Furthermore, the acinar-like cell clusters express REG Iα protein and secrete directly to neighboring islet endocrine cells in the non-diabetic state, and the cell clusters over-express REG Iα in the inflamed milieu of fulminant type 1 diabetes with marked self-replication of islet cells.
登录
查看更多内容
影响因子：
7.7
作者：
Korpos É;Kadri N;Kappelhoff R;Wegner J;Overall CM;Weber E;Holmberg D;Cardell S;Sorokin L
通讯作者：
Sorokin L
影响因子：
4.8
作者：
Kobayashi, S;Akiyama, T;Okamoto, H
通讯作者：
Okamoto, H
影响因子：
1.4
作者：
Agarwal, Avinash;Brayman, Kenneth L
通讯作者：
Brayman, Kenneth L
影响因子：
2.2
作者：
Mueller, Cathy M.;Zhang, Hong;Zenilman, Michael E.
通讯作者：
Zenilman, Michael E.
DOI：
10.1007/978-90-481-3271-3_10
发表时间：
2010-01-01
期刊：
ISLETS OF LANGERHANS
影响因子：
--
作者：
Kragl, Martin;Lammert, Eckhard
通讯作者：
Lammert, Eckhard