Collecting mouse livers for transcriptome analysis of daily rhythms.
Collecting mouse livers for transcriptome analysis of daily rhythms.
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DOI：
10.1016/j.xpro.2021.100539
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Koronowski KB
中科院分区：
文献类型：
--
作者：
Mortimer T;Welz PS;Benitah SA;Koronowski KB
分子的日常节律可以通过精确定时的动物组织收获来捕获。该方案将允许研究人员识别小鼠肝脏中的转录节律，同时还为其他整个代谢器官中的类似分析提供模板。我们描述了从总RNA测序数据中进行小鼠夹带、肝脏解剖和节律性分析的步骤。由此产生的节律转录组将为用户提供定义经历日常节律的特定生物过程的起点。有关本方案使用和执行的完整详细信息，请参阅。一个类似的方案，滤泡间表皮细胞中证明。在第四维度时间中研究基因或途径使用精确定时的组织收获来检测每日转录节律小鼠夹带、肝脏解剖和节律性分析的完整描述用于研究其他整个代谢器官的参考方案分子每日节律可以通过精确定时的动物组组织收获来捕获。该方案将允许研究人员识别小鼠肝脏中的转录节律，同时还为其他整个代谢器官中的类似分析提供模板。我们描述了从总RNA测序数据中进行小鼠夹带、肝脏解剖和节律性分析的步骤。由此产生的节律转录组将为用户提供定义经历日常节律的特定生物过程的起点。
Molecular daily rhythms can be captured by precisely timed tissue harvests from groups of animals. This protocol will allow the investigator to identify transcriptional rhythms in the mouse liver while also providing a template for similar analyses in other whole metabolic organs. We describe steps for mouse entrainment, liver dissection, and rhythmicity analysis from total RNA sequencing data. The resulting rhythmic transcriptome will provide the user with a starting point for defining specific biological processes that undergo daily rhythms. For complete details on the use and execution of this protocol, please refer to. A similar protocol for interfollicular epidermal cells is demonstrated in. Investigate a gene or pathway in the fourth dimension—time Use precisely timed tissue harvest to detect daily transcriptional rhythms Full description of mouse entrainment, liver dissection, and rhythmicity analysis A reference protocol for investigating other whole metabolic organs Molecular daily rhythms can be captured by precisely timed tissue harvests from groups of animals. This protocol will allow the investigator to identify transcriptional rhythms in the mouse liver while also providing a template for similar analyses in other whole metabolic organs. We describe steps for mouse entrainment, liver dissection, and rhythmicity analysis from total RNA sequencing data. The resulting rhythmic transcriptome will provide the user with a starting point for defining specific biological processes that undergo daily rhythms.
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