Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep.
Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep.
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网格蛋白重链 1 在绵羊卵母细胞减数分裂纺锤体形成和早期胚胎发育过程中发挥重要作用
DOI：
10.3389/fcell.2021.609311
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发表时间：
2021
影响因子：
5.5
通讯作者：
Liang CG
中科院分区：
文献类型：
--
作者：
Han Z;Hao X;Zhou CJ;Wang J;Wen X;Wang XY;Zhang DJ;Liang CG
网格蛋白作为被膜小凹和囊泡的多面体外壳的主要蛋白质，已被证明通过充当微管间的桥而对有丝分裂纺锤体的动粒纤维起稳定作用。网格蛋白重链1（CLTC）是网格蛋白外壳的基本亚基，在体细胞有丝分裂过程中对纺锤体组装和染色体聚集起着重要作用。然而，其在哺乳动物，特别是家养动物卵母细胞减数分裂成熟和早期胚胎发育中的功能尚未得到充分研究。本研究对CLTC基因在绵羊卵母细胞中的表达谱及其功能进行了研究。结果表明，CLTC在胚泡（GV）期至中期II期维持高水平表达，在GV期至囊胚期的间期细胞胞质中呈弥散分布。GV分裂（GV breakdown，GVBD）后，CLTC在分裂中期与β-微管蛋白共定位。卵母细胞处理紫杉醇，诺考达唑，或冷不影响CLTC的表达水平，但导致其分布的障碍。特异性吗啉注射GV期卵母细胞导致CLTC功能受损，导致纺锤体组装和染色体排列中断，伴有第一极体（PB 1）发射受损。此外，在孤雌激活前敲低CLTC会破坏纺锤体的形成并损害早期胚胎的发育。这些结果表明，CLTC通过调节纺锤体动力学在绵羊卵母细胞成熟中起着至关重要的作用，并在早期胚胎发育中起着重要作用。
As a major protein of the polyhedral coat of coated pits and vesicles, clathrin molecules have been shown to play a stabilization role for kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridges. Clathrin heavy chain 1 (CLTC), the basic subunit of the clathrin coat, plays vital roles in both spindle assembly and chromosome congression during somatic-cell mitosis. However, its function in oocyte meiotic maturation and early embryo development in mammals, especially in domesticated animals, has not been fully investigated. In this study, the expression profiles and functional roles of CLTC in sheep oocytes were investigated. Our results showed that the expression of CLTC was maintained at a high level from the germinal vesicle (GV) stage to metaphase II stage and that CLTC was distributed diffusely in the cytoplasm of cells at interphase, from the GV stage to the blastocyst stage. After GV breakdown (GVBD), CLTC co-localized with beta-tubulin during metaphase. Oocyte treatments with taxol, nocodazole, or cold did not affect CLTC expression levels but led to disorders of its distribution. Functional impairment of CLTC by specific morpholino injections in GV-stage oocytes led to disruptions in spindle assembly and chromosomal alignment, accompanied by impaired first polar body (PB1) emissions. In addition, knockdown of CLTC before parthenogenetic activation disrupted spindle formation and impaired early embryo development. Taken together, the results demonstrate that CLTC plays a vital role in sheep oocyte maturation via the regulation of spindle dynamics and an essential role during early embryo development.
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影响因子：
16.6
作者：
Petry S
通讯作者：
Petry S
影响因子：
8
作者：
Fielding, Andrew B.;Royle, Stephen J.
通讯作者：
Royle, Stephen J.
影响因子：
3.3
作者：
Radulescu, Andreea E.;Siddhanta, Anirban;Shields, Dennis
通讯作者：
Shields, Dennis
影响因子：
4.8
作者：
Dionne, MA;Sanchez, A;Compton, DA
通讯作者：
Compton, DA
网格蛋白将磷酸化的 TACC3 招募到纺锤体极，以进行双极纺锤体组装和染色体排列
影响因子：
4
作者：
Fu, Wenxiang;Tao, Wei;Zhang, Chuanmao
通讯作者：
Zhang, Chuanmao