Dynamic changes in the expression of MicroRNA-31 during inflammatory bowel disease-associated neoplastic transformation.
Dynamic changes in the expression of MicroRNA-31 during inflammatory bowel disease-associated neoplastic transformation.
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DOI：
10.1002/ibd.21359
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Meltzer, Stephen J.
中科院分区：
文献类型：
--
作者：
Olaru, Alexandru V.;Selaru, Florin M.;Mori, Yuriko;Vazquez, Christine;David, Stefan;Paun, Bogdan;Cheng, Yulan;Jin, Zhe;Yang, Jian;Agarwal, Rachana;Abraham, John M.;Dassopoulos, Themistocles;Harris, Mary;Bayless, Theodore M.;Kwon, John;Harpaz, Noam;Livak, Ferenc;Meltzer, Stephen J.
关键词：
炎症性肠病（IBD）患者患结直肠癌的风险增加。异常microRNA（miR）表达与癌发生有关，然而没有报告记录IBD相关瘤形成（IBDN）与改变的miR表达之间的关系。在目前的研究中，我们试图确定特定的miR失调沿着正常炎症癌轴。miR微阵列和定量RT-PCR用于检测失调的miR。采用受试者操作特征曲线分析来测试miR-31作为IBDN的疾病标志物的潜在有用性。采用计算机模拟预测分析、蛋白质印迹和荧光素酶活性测量进行靶标鉴定。在IBD中出现的慢性炎症粘膜和发育不良之间鉴定出几种失调的miR。MiR-31表达在从正常到IBD到IBDN的进展期间以逐步方式增加，并且准确地将IBD患者中的IBDN与正常或慢性炎症组织区分开。最后，我们确定了抑制缺氧诱导因子1的因子作为miR-31的直接靶点。我们的研究揭示了随着慢性炎症进展为异型增生，特定的miR失调。miR-31表达水平随着疾病进展而增加，并准确区分IBD患者中共存的不同病理实体。miR-31在抑制缺氧诱导因子1表达的调节因子中的新作用为IBDN的发病机制提供了新的见解。
Patients with inflammatory bowel disease (IBD) are at increased risk of developing colorectal cancer. Aberrant microRNA (miRs) expression has been linked to carcinogenesis, however no reports document a relationship between IBD-related neoplasia (IBDN) and altered miR expression. In the current study we sought to identify specific miR dysregulation along the normal-inflammation-cancer axis. miR microarrays and quantitative RT-PCR were used to detect dysregulated miRs. Receiver operating characteristic curve analysis was employed to test for potential usefulness of miR-31 as a disease marker of IBDNs. In silico prediction analysis, Western blot, and luciferase activity measurement were employed for target identification. Several dysregulated miRs were identified between chronically inflamed mucosae and dysplasia arising in IBD. MiR-31 expression increases in a stepwise fashion during progression from normal to IBD to IBDN and accurately discriminated IBDNs from normal or chronically inflamed tissues in IBD patients. Finally, we identified factor inhibiting hypoxia inducible factor 1 as a direct target of miR-31. Our study reveals specific miR dysregulation as chronic inflammation progresses to dysplasia. MiR-31 expression levels increase with disease progression and accurately discriminates between distinct pathological entities that co-exist in IBD patients. The novel effect of miR-31 on regulating factor inhibiting hypoxia inducible factor 1 expression provides a new insight on the pathogenesis of IBDN.
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影响因子：
11.2
作者：
Liu, Chung-Ji;Tsai, Meng-Miao;Chang, Kuo-Wei
通讯作者：
Chang, Kuo-Wei
DOI：
10.1073/pnas.0602266103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Costinean, S;Zanesi, N;Croce, CM
通讯作者：
Croce, CM
影响因子：
37.3
作者：
Bandrés E;Cubedo E;Agirre X;Malumbres R;Zárate R;Ramirez N;Abajo A;Navarro A;Moreno I;Monzó M;García-Foncillas J
通讯作者：
García-Foncillas J
影响因子：
29.4
作者：
Wu, Feng;Zikusoka, Michelle;Kwon, John H.
通讯作者：
Kwon, John H.
影响因子：
7.3
作者：
Eaden, J;Abrams, K;Mayberry, J
通讯作者：
Mayberry, J