Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
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DOI：
10.1186/1476-4598-5-29
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发表时间：
2006-07-19
期刊：
影响因子：
37.3
通讯作者：
García-Foncillas J
中科院分区：
文献类型：
--
作者：
Bandrés E;Cubedo E;Agirre X;Malumbres R;Zárate R;Ramirez N;Abajo A;Navarro A;Moreno I;Monzó M;García-Foncillas J
微小RNA（miRNAs）是短的非编码RNA分子，通过抑制翻译或切割RNA转录本发挥调节作用。尽管已验证的人类miRNA数量仍在增加，但只有少数已在功能上得到描述。然而，新出现的证据表明，miRNA调控的改变可能参与癌症的发病机制，并且这些基因被认为既具有肿瘤抑制基因的功能，又具有癌基因的功能。
在我们的研究中，我们通过实时定量聚合酶链反应（Real - Time PCR）检测了156种成熟miRNA在结直肠癌中的表达。对来自患者的结直肠肿瘤及相邻的非肿瘤组织以及结直肠癌细胞系进行的几种生物信息学算法分析，确定了一组13种在该肿瘤中表达显著改变的miRNA。表达失调最显著的miRNA是miR - 31、miR - 96、miR - 133b、miR - 135b、miR - 145和miR - 183。此外，miR - 31的表达水平与结直肠癌（CRC）肿瘤的分期相关。
我们的结果表明，miRNA表达谱可能与结直肠肿瘤的生物学和临床行为相关。
MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles by repressing translation or cleaving RNA transcripts. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the potential involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. In our study, we examined by Real-Time PCR the expression of 156 mature miRNA in colorectal cancer. The analysis by several bioinformatics algorithms of colorectal tumours and adjacent non-neoplastic tissues from patients and colorectal cancer cell lines allowed identifying a group of 13 miRNA whose expression is significantly altered in this tumor. The most significantly deregulated miRNA being miR-31, miR-96, miR-133b, miR-135b, miR-145, and miR-183. In addition, the expression level of miR-31 was correlated with the stage of CRC tumor. Our results suggest that miRNA expression profile could have relevance to the biological and clinical behavior of colorectal neoplasia.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
64.8
作者：
O'Donnell, KA;Wentzel, EA;Mendell, JT
通讯作者：
Mendell, JT
影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM
DOI：
10.1073/pnas.0403293101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Liu, CG;Calin, GA;Croce, CM
通讯作者：
Croce, CM