High expression of high mobility group box 1 (hmgb1) predicts poor prognosis for hepatocellular carcinoma after curative hepatectomy.
High expression of high mobility group box 1 (hmgb1) predicts poor prognosis for hepatocellular carcinoma after curative hepatectomy.
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DOI：
10.1186/1479-5876-10-135
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发表时间：
2012-07-02
影响因子：
7.4
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Liu F;Zhang Y;Peng Z;Gao H;Xu L;Chen M
高迁移率族蛋白1（HMGB 1）过表达已在多种人类癌症中报道。然而，HMGB 1在肝细胞癌（HCC）中的作用仍不清楚。本研究的目的是分析HMGB 1蛋白在肝癌、癌旁和正常组织中的表达，并评估其对肝癌根治性切除术后预后的意义。应用逆转录-聚合酶链反应（RT-PCR）和Western blot检测11例肝癌组织、癌旁组织和正常肝组织中HMGB 1 mRNA和蛋白的表达。此外，HMGB 1蛋白表达与161 HCC的临床病理特征和生存率进行了分析，通过免疫组化。采用t检验、等级相关、Kaplan-Meier曲线和考克斯比例风险回归模型对数据进行分析。RT-PCR和Westernblot检测结果显示，HMGB 1 mRNA和蛋白在肝癌组织中的表达均显著高于癌旁组织（p < 0.001）和正常肝组织（p < 0.001）。免疫组化结果显示HMGB 1在42.9%（69/161）的HCC组织中呈高表达。HMGB 1的高表达与包膜不完全（p = 0.035）和晚期TNM分期（p = 0.036）显著相关。多因素分析显示HMGB 1高表达是影响总生存期（p = 0.009，HR = 1.834，95%CI：1.167-2.881）和无病生存期（p = 0.018，HR = 1.622，95%CI：1.088-2.419）的独立预后因素，沿着肿瘤大小。亚组分析显示，HMGB 1高表达仅预测肿瘤>5 cm的总生存率较差（p = 0.031），但不预测肿瘤≤5 cm的总生存率（p = 0.101）。HMGB 1蛋白可能参与了肝癌的恶性进展，HMGB 1高表达预示肝癌根治性切除术后预后不良，尤其是肿瘤> 5cm的患者。
High mobility group box 1(HMGB1) overexpression has been reported in a variety of human cancers. However, the role of HMGB1 in hepatocellular carcinoma (HCC) remains unclear. The aim of present study was to analyze HMGB1 protein expression in tumor, para-tumor and normal tissue and to assess its prognostic significance for HCC after curative hepatectomy. The levels of HMGB1 mRNA and protein in tumor, para-tumor and normal tissue were evaluated in 11 HCC cases by Reverse Transcription-polymerase chain reaction (RT-PCR) and Western blot. Additionally, HMGB1 protein expression in 161 HCC was analyzed by immunohistochemistry and correlated with clinicopathological characteristics and survivals. Student’s t-test, spearman’s rank correlation, Kaplan-Meier plots and Cox proportional hazards regression model were used to analyze the data. By RT-PCR and Western blot, the levels of HMGB1 mRNA and protein were significantly higher in HCC, compared to that in para-tumor (p < 0.001) and normal tissue (p < 0.001). Immunohistochemical staining revealed that high expression of HMGB1 was detected in 42.9% (69/161) HCC cases. High expression of HMGB1 was significantly associated with incomplete encapsulation (p = 0.035) and advanced TNM stage (p = 0.036). Multivariate analysis showed that high expression of HMGB1 was an independent prognostic factor for both overall (p = 0.009, HR = 1.834, 95%CI: 1.167-2.881) and disease-free survival (p = 0.018, HR = 1.622, 95%CI: 1.088-2.419), along with tumor size. Subgroup analysis revealed that high expression of HMGB1 predicted poorer overall survival only for tumor >5 cm (p = 0.031), but not for tumor ≤5 cm (p = 0.101). HMGB1 protein might contribute to the malignant progression of HCC, high expression of HMGB1 predicts poor prognosis for patients with HCC after curative hepatectomy, especially for patients with tumor >5 cm.
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影响因子：
64.8
作者：
Scaffidi, P;Misteli, T;Bianchi, ME
通讯作者：
Bianchi, ME
影响因子：
2.8
作者：
Luan, Zheng-Gang;Zhang, Hao;Guo, Ren-Xuan
通讯作者：
Guo, Ren-Xuan
高迁移率族蛋白 1 (HMGB1) 表达增加与人鼻咽癌的进展和不良预后相关。
影响因子：
7.3
作者：
Wu, D.;Ding, Y.;Liu, L.
通讯作者：
Liu, L.
影响因子：
50.3
作者：
Bruix, J;Boix, L;Llovet, JM
通讯作者：
Llovet, JM
DOI：
10.1007/s00432-009-0706-1
发表时间：
2010-05-01
影响因子：
3.6
作者：
Yao, Xingjun;Zhao, Gang;Liu, Guojin
通讯作者：
Liu, Guojin