Uncovering viral RNA-host cell interactions on a proteome-wide scale.
Uncovering viral RNA-host cell interactions on a proteome-wide scale.
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在蛋白质组范围内揭示病毒RNA与宿主细胞的相互作用。
DOI：
10.1016/j.tibs.2021.08.002
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发表时间：
2022-01
影响因子：
13.8
通讯作者：
Castello, Alfredo
中科院分区：
文献类型：
--
作者：
Iselin, Louisa;Palmalux, Natasha;Kamel, Wael;Simmonds, Peter;Mohammed, Shabaz;Castello, Alfredo
RNA病毒在其生命周期中与多种细胞RNA结合蛋白（RBP）相互作用。阐明病毒核糖核蛋白（vRNP）组成的新方法强调了这些宿主-病毒相互作用的普遍性。到目前为止，这些方法已经应用于11种病毒，揭示了数百种与感染细胞中的病毒（v）RNA相互作用的细胞RBP。然而，方法之间的一致性有限，这引发了设计和解释这些研究时方法学考虑的问题。在这里，我们讨论了这些警告，并通过比较可用的vRNA相互作用组，描述了一致确定为vRNP组件的RBP，并概述了它们在感染中的潜在作用。总之，这些新方法揭示了宿主-病毒相互作用的新领域，具有巨大的治疗潜力。RNA病毒在其生命周期中与许多RNA结合蛋白（RBP）相互作用，具有促病毒和抗病毒能力。病毒（v）RNA-宿主RBP相互作用的真实程度直到最近才变得清晰，这是由于质谱法和专门研究RBP的方法的发展。现在有几种方法可用于直接研究vRNA相互作用组;这些方法使用不同的方法来特异性捕获与vRNA相互作用的蛋白质，并且通常产生非常不同的结果。当设计良好和正确的解释，这些方法有可能大大提高我们对病毒感染的理解，通过提供一个公正的洞察病毒核糖核蛋白的组成。在多种病毒的vRNA相互作用组中鉴定的蛋白质可能具有泛病毒调节作用，并且可能是开发广谱抗病毒疗法的有希望的靶点。
RNA viruses interact with a wide range of cellular RNA-binding proteins (RBPs) during their life cycle. The prevalence of these host–virus interactions has been highlighted by new methods that elucidate the composition of viral ribonucleoproteins (vRNPs). Applied to 11 viruses so far, these approaches have revealed hundreds of cellular RBPs that interact with viral (v)RNA in infected cells. However, consistency across methods is limited, raising questions about methodological considerations when designing and interpreting these studies. Here, we discuss these caveats and, through comparing available vRNA interactomes, describe RBPs that are consistently identified as vRNP components and outline their potential roles in infection. In summary, these novel approaches have uncovered a new universe of host–virus interactions holding great therapeutic potential. RNA viruses interact with many RNA-binding proteins (RBPs) over the course of their life cycle in both pro- and antiviral capacities. The true extent of viral (v)RNA–host RBP interactions has only recently become clear as a result of developments in mass spectrometry and methods for specifically studying RBPs. There are now several methods available for directly studying the vRNA interactome; these use different approaches to specifically capture proteins interacting with vRNA and often yield very different results. When well designed and correctly interpreted, these methods have the potential to greatly enhance our understanding of viral infection by providing an unbiased insight into viral ribonucleoprotein composition. Proteins identified in the vRNA interactomes of multiple viruses might have panviral regulatory roles and could be promising targets for developing broad-spectrum antiviral therapies.
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影响因子：
4.4
作者：
Asencio C;Chatterjee A;Hentze MW
通讯作者：
Hentze MW
影响因子：
3.7
作者：
Abernathy, Emma;Glaunsinger, Britt
通讯作者：
Glaunsinger, Britt
影响因子：
16
作者：
Caudron-Herger, Maiwen;Rusin, Scott F.;Diederichs, Sven
通讯作者：
Diederichs, Sven
影响因子：
5
作者：
Charley PA;Wilusz J
通讯作者：
Wilusz J
DOI：
10.1016/s0006-291x(86)80250-9
发表时间：
1986-12-15
影响因子：
3.1
作者：
FAVRE, A;MORENO, G;SALET, C
通讯作者：
SALET, C