Salt appetite is reduced by a single experience of drinking hypertonic saline in the adult rat.
Salt appetite is reduced by a single experience of drinking hypertonic saline in the adult rat.
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DOI：
10.1371/journal.pone.0104802
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Murphy D
中科院分区：
文献类型：
--
作者：
Greenwood MP;Greenwood M;Paton JF;Murphy D
盐的食欲是一种有利于摄取含盐物质的原始本能，是成功维持体液和电解质平衡的重要进化动力。这种天生的本能在Sprague-Dawley大鼠身上得到了体现，在液体摄入试验中，等渗盐水（IS）的摄取量高于水。然而，在摄入测试中，通过将饮用液体的含盐量从IS增加到高渗盐水（2% w/v NaCl， HS），这种食欲刺激从根本上转变为强烈的厌恶刺激。在单瓶试验中，当没有选择时，大鼠摄入的HS与IS相似，先前的研究表明，这可能会改变盐的食欲。因此，我们调查了单次摄入IS或HS、脱水（DH）或4%高盐食物（HSD） 24小时是否会改变盐偏好。本研究表明，在7天和35天后，摄入IS和HS溶液24 h，而不是DH或HSD，可以显著改变大鼠的盐食欲。通过两瓶试验，先前暴露于IS的大鼠既不喜欢IS也不喜欢水，而暴露于HS的大鼠则对IS表现出厌恶。对甜味溶液（1%蔗糖）的反应在两瓶水的测试中没有什么不同，这表明盐是该模型中招募的主要厌恶味觉途径。通过皮下注射血管紧张素II诱导口渴并不能克服这种对盐的厌恶。我们假设这种行为是由于大脑结构中对口渴和盐的食欲很重要的基因表达改变造成的。因此，我们在这里也报道了下丘脑视上核和室旁核在DH和HS后补液后神经元活性、肽激素和神经元可塑性标志物mrna的持续变化。这些结果表明，饮用HS的一次经历是令人难忘的，长期的基因表达变化伴随着这种对咸溶液的厌恶。
Salt appetite, the primordial instinct to favorably ingest salty substances, represents a vital evolutionary important drive to successfully maintain body fluid and electrolyte homeostasis. This innate instinct was shown here in Sprague-Dawley rats by increased ingestion of isotonic saline (IS) over water in fluid intake tests. However, this appetitive stimulus was fundamentally transformed into a powerfully aversive one by increasing the salt content of drinking fluid from IS to hypertonic saline (2% w/v NaCl, HS) in intake tests. Rats ingested HS similar to IS when given no choice in one-bottle tests and previous studies have indicated that this may modify salt appetite. We thus investigated if a single 24 h experience of ingesting IS or HS, dehydration (DH) or 4% high salt food (HSD) altered salt preference. Here we show that 24 h of ingesting IS and HS solutions, but not DH or HSD, robustly transformed salt appetite in rats when tested 7 days and 35 days later. Using two-bottle tests rats previously exposed to IS preferred neither IS or water, whereas rats exposed to HS showed aversion to IS. Responses to sweet solutions (1% sucrose) were not different in two-bottle tests with water, suggesting that salt was the primary aversive taste pathway recruited in this model. Inducing thirst by subcutaneous administration of angiotensin II did not overcome this salt aversion. We hypothesised that this behavior results from altered gene expression in brain structures important in thirst and salt appetite. Thus we also report here lasting changes in mRNAs for markers of neuronal activity, peptide hormones and neuronal plasticity in supraoptic and paraventricular nuclei of the hypothalamus following rehydration after both DH and HS. These results indicate that a single experience of drinking HS is a memorable one, with long-term changes in gene expression accompanying this aversion to salty solutions.
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影响因子：
15.9
作者：
Korb E;Finkbeiner S
通讯作者：
Finkbeiner S
影响因子：
5.3
作者：
CHAN, RKW;BROWN, ER;SAWCHENKO, PE
通讯作者：
SAWCHENKO, PE
DOI：
10.1152/ajpregu.00582.2003
发表时间：
2004-06-01
影响因子：
2.8
作者：
Curtis, KS;Krause, EG;Contreras, RJ
通讯作者：
Contreras, RJ
影响因子：
2.5
作者：
ARNAULD, E;WATTIAUX, JP;VINCENT, JD
通讯作者：
VINCENT, JD
影响因子：
2.9
作者：
Curtis, KS;Krause, EG;Contreras, RJ
通讯作者：
Contreras, RJ