A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT).
A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT).
复制标题
DOI：
10.1083/jcb.141.4.979
复制
发表时间：
1998-05-18
影响因子：
7.8
通讯作者：
Witman, GB
中科院分区：
文献类型：
--
作者：
Pazour, GJ;Wilkerson, CG;Witman, GB
几种酶，包括细胞质和鞭毛外臂动力蛋白，共有一个Mr 8，000轻链，称为LC 8。该链的功能尚不清楚，但它在各种生物体之间高度保守。我们已经确定了缺失等位基因的基因（fla 14）编码这种蛋白质在莱茵衣藻。这些突变体有短，不动的鞭毛的缺陷，在径向辐条，在内部和外部武器，并在喙状突起的B微管的外部双重微管。最引人注目的是，双微管和鞭毛膜之间的空间包含异常高数量的筏，颗粒通过鞭毛内运输（IFT）移位（Kozminski，K.G.，P.L. Beech和J.L.罗森鲍姆1995. 131：1517-1527）。IFT是鞭毛膜下的筏沿着轴丝微管的快速双向运动。顺行性IFT依赖于驱动蛋白，而逆行性IFT的马达尚不清楚。顺行IFT在LC 8突变体中是正常的，但逆行IFT不存在;这无疑是鞭毛中筏积累的原因。这是第一个显示出特异性影响逆行IFT的突变; LC 8缺失影响逆行IFT的事实强烈表明细胞质动力蛋白是驱动这一过程的马达。伴随着筏的积累，LC 8突变体积累作为15- 16 S IFT复合物的组分的蛋白质（科尔，D.G.，D.R. Deiner，A.L. Himelblau，P.L.比奇，J.C. Fuster和J.L.罗森鲍姆1998. J. Cell Biol.141：993-1008），证实了这些复合物是筏的亚基。聚苯乙烯微珠仍然易位的表面上的鞭毛的LC 8突变体，表明鞭毛表面运动的电机是不同的电机逆行IFT。
Several enzymes, including cytoplasmic and flagellar outer arm dynein, share an M r 8,000 light chain termed LC8. The function of this chain is unknown, but it is highly conserved between a wide variety of organisms. We have identified deletion alleles of the gene (fla14) encoding this protein in Chlamydomonas reinhardtii. These mutants have short, immotile flagella with deficiencies in radial spokes, in the inner and outer arms, and in the beak-like projections in the B tubule of the outer doublet microtubules. Most dramatically, the space between the doublet microtubules and the flagellar membrane contains an unusually high number of rafts, the particles translocated by intraflagellar transport (IFT) (Kozminski, K.G., P.L. Beech, and J.L. Rosenbaum. 1995. J. Cell Biol. 131:1517–1527). IFT is a rapid bidirectional movement of rafts under the flagellar membrane along axonemal microtubules. Anterograde IFT is dependent on a kinesin whereas the motor for retrograde IFT is unknown. Anterograde IFT is normal in the LC8 mutants but retrograde IFT is absent; this undoubtedly accounts for the accumulation of rafts in the flagellum. This is the first mutation shown to specifically affect retrograde IFT; the fact that LC8 loss affects retrograde IFT strongly suggests that cytoplasmic dynein is the motor that drives this process. Concomitant with the accumulation of rafts, LC8 mutants accumulate proteins that are components of the 15-16S IFT complexes (Cole, D.G., D.R. Deiner, A.L. Himelblau, P.L. Beech, J.C. Fuster, and J.L. Rosenbaum. 1998. J. Cell Biol. 141:993–1008), confirming that these complexes are subunits of the rafts. Polystyrene microbeads are still translocated on the surface of the flagella of LC8 mutants, indicating that the motor for flagellar surface motility is different than the motor for retrograde IFT.
登录
查看更多内容
DOI：
10.1073/pnas.86.17.6449
发表时间：
1989-09-01
影响因子：
11.1
作者：
FERNANDEZ, E;SCHNELL, R;LEFEBVRE, PA
通讯作者：
LEFEBVRE, PA
影响因子：
19
作者：
Gumpel, Nicola J.;Purton, Saul
通讯作者：
Purton, Saul
DOI：
10.1083/jcb.97.3.902
发表时间：
1983-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoops HJ;Witman GB
通讯作者：
Witman GB
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL
影响因子：
4.8
作者：
Benashski, SE;Harrison, A;King, SM
通讯作者：
King, SM