Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
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DOI：
10.1083/jcb.141.4.993
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发表时间：
1998-05-18
期刊：
影响因子：
--
通讯作者：
Rosenbaum JL
中科院分区：
文献类型：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
我们先前描述了被称为鞭毛内转运（IFT）的莱茵衣原体鞭毛中的颗粒的驱动蛋白依赖性运动（Kozminski，K.G.，K.A.约翰逊、P.福舍尔和J.L.罗森鲍姆1993. Proc. Natl. Acad. Sci. USA. 90：5519-5523）。当IFT抑制失活的驱动蛋白，FLA 10，在温度敏感的突变体，fla 10，现有的鞭毛resorb和新的鞭毛不能组装。我们在此报告：（a）IFT相关的FLA 10蛋白是异源三聚体驱动蛋白的亚基;（B）IFT颗粒由包含两个大复合物的15种多肽组成;（c）FLA 10驱动蛋白-II和IFT颗粒多肽除了在鞭毛中发现之外，还高度集中在鞭毛基体周围;和（d）影响秀丽隐杆线虫中两种IFT颗粒多肽的同源物的突变导致位于感觉神经元的树突上的感觉纤毛的缺陷。在Pazour，G. J.的随附报告中，C.G. Wilkerson和G.B. Witman（1998年。J. Cell Biol.141：979-992），描述了一种衣原体突变体（fla 14），其中仅IFT颗粒的逆行转运被破坏，导致装配缺陷的鞭毛充满过量的IFT颗粒。这种微管依赖的运输过程，IFT，定义为可分离颗粒的顺行（fla 10）和逆行（fla 14）运输的突变体，可能是所有真核生物能动鞭毛和非能动感觉纤毛的维持和组装所必需的。
We previously described a kinesin-dependent movement of particles in the flagella of Chlamydomonas reinhardtii called intraflagellar transport (IFT) (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. USA. 90:5519–5523). When IFT is inhibited by inactivation of a kinesin, FLA10, in the temperature-sensitive mutant, fla10, existing flagella resorb and new flagella cannot be assembled. We report here that: (a) the IFT-associated FLA10 protein is a subunit of a heterotrimeric kinesin; (b) IFT particles are composed of 15 polypeptides comprising two large complexes; (c) the FLA10 kinesin-II and IFT particle polypeptides, in addition to being found in flagella, are highly concentrated around the flagellar basal bodies; and, (d) mutations affecting homologs of two of the IFT particle polypeptides in Caenorhabditis elegans result in defects in the sensory cilia located on the dendritic processes of sensory neurons. In the accompanying report by Pazour, G.J., C.G. Wilkerson, and G.B. Witman (1998. J. Cell Biol. 141:979–992), a Chlamydomonas mutant (fla14) is described in which only the retrograde transport of IFT particles is disrupted, resulting in assembly-defective flagella filled with an excess of IFT particles. This microtubule- dependent transport process, IFT, defined by mutants in both the anterograde (fla10) and retrograde (fla14) transport of isolable particles, is probably essential for the maintenance and assembly of all eukaryotic motile flagella and nonmotile sensory cilia.
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