Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactions.
Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactions.
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DOI：
10.1016/j.cell.2011.03.053
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发表时间：
2011-05-27
期刊：
影响因子：
64.5
通讯作者：
Allis CD
中科院分区：
文献类型：
--
作者：
Ruthenburg AJ;Li H;Milne TA;Dewell S;McGinty RK;Yuen M;Ueberheide B;Dou Y;Muir TW;Patel DJ;Allis CD
关于组蛋白标记的组合如何在核小体的水平上被解释，我们知之甚少。已知人BPTF的第二个PHD指在赖氨酸4（H3 K4 me 2/3）上甲基化时特异性识别组蛋白H3;在这里，我们研究额外的异型修饰如何影响BPTF结合。使用肽替代物，通过系统筛选和生物物理表征，确定了BPTF中PH邻近溴结构域的三个乙酰赖氨酸配体。尽管溴结构域在肽水平上在三种可能的乙酰基赖氨酸之间显示有限的区分，但在单核苷酸水平上观察到这些位点中仅一个H4 K16 ac与H3 K4 me 3组合的显著选择性。作为支持，这两个组蛋白标记构成了独特的反式组蛋白修饰模式，其明确地存在于人类细胞中的单个核小体单元内，并且该模块与基因组中的这些标记共定位。总之，我们的数据呼吁注意核小体图案的共价标记在决定关键的染色质协会。
Little is known about how combinations of histone marks are interpreted at the level of nucleosomes. The second PHD finger of human BPTF is known to specifically recognize histone H3 when methylated on lysine 4 (H3K4me2/3); here we examine how additional heterotypic modifications influence BPTF binding. Using peptide surrogates, three acetyllysine ligands are indentified for a PHD-adjacent bromodomain in BPTF via systematic screening and biophysical characterization. Although the bromodomain displays limited discrimination amongst the three possible acetyllysines at the peptide level, marked selectivity is observed for only one of these sites, H4K16ac, in combination with H3K4me3 at the mononucleosome level. In support, these two histone marks constitute a unique trans-histone modification pattern that unambiguously resides within a single nucleosomal unit in human cells, and this module co-localizes with these marks in the genome. Together, our data call attention to nucleosomal patterning of covalent marks in dictating critical chromatin associations.
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影响因子：
14.8
作者：
Brand, Marjorie;Rampalli, Shravanti;Dilworth, F. Jeffrey
通讯作者：
Dilworth, F. Jeffrey
影响因子：
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作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
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影响因子：
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作者：
Grüne, T;Brzeski, J;Müller, CW
通讯作者：
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影响因子：
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作者：
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通讯作者：
Shiekhattar, R
DOI：
10.1073/pnas.0906801106
发表时间：
2009-08-18
影响因子：
11.1
作者：
Basu, Amrita;Rose, Kristie L.;Hake, Sandra B.
通讯作者：
Hake, Sandra B.