Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies
Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies
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溶质在琼脂糖和藻酸盐凝胶中的扩散：1H 和 23Na PFGSE 以及 23Na TQF NMR 研究
DOI：
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发表时间：
1997
影响因子：
3.3
通讯作者：
P. Kuchel
中科院分区：
文献类型：
--
作者：
P. Lundberg;P. Kuchel
固定在凝胶中的细胞经历潜在的代谢限制，其形式为代谢物和离子的扩散速率降低以及它们在凝胶基质上的可能的选择性吸附。用1H PFGSE和23Na TQF NMR研究了37°C下常见溶质在琼脂糖和海藻酸钡凝胶中的扩散和弛豫特性。研究了葡萄糖、甘氨酸、丙氨酸、乳酸盐、钠离子和HDO。任何代谢物和凝胶材料之间均无选择性相互作用，但扩散系数均匀降低。代谢产物的扩散和利用的影响，在凝胶珠和线程含有细胞，模拟通过使用反应扩散模型，将测得的扩散系数。预计代谢将受到溶质扩散至和来自位于半径为2.0 mm（通常使用的尺寸）的凝胶线或球体中心的细胞的非常显著的限制。
Cells immobilized in gels experience potential metabolic restrictions in the form of reduced diffusion rates of metabolites and ions and their possible selective adsorption on the gel matrix. Diffusion and relaxation characteristics of common solutes in agarose and barium alginate gels were investigated at 37°C by using 1H PFGSE and 23Na TQF NMR spectroscopy. Glucose, glycine, alanine, lactate, sodium ions, and HDO were studied. There were no selective interactions between any of the metabolites and the gel materials but the diffusion coefficients were uniformly reduced. The effects of metabolite diffusion and utilization, in gel beads and threads containing cells, were simulated by using a reaction diffusion model incorporating the measured diffusion coefficients. Metabolism is expected to be very significantly limited by diffusion of solutes to and from the cells that are centrally located within gel threads or spheres of radius ∼2.0 mm, which is a cornmonly used size.
通过一维多量子过滤简化蛋白质 1H NMR 谱。
DOI：
10.1016/0006-291x(85)90203-7
发表时间：
1985
影响因子：
3.1
作者：
Rance,M;Dalvit,C;Wright,PE
通讯作者：
Wright,PE
固定在琼脂糖线中的灌注大鼠肝细胞的 31P-NMR 光谱：应用于化学诱导的肝毒性。
DOI：
10.1016/0925-4439(92)90089-6
发表时间：
1992
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Farghali,H;Rossaro,L;Gavaler,JS;VanThiel,DH;Dowd,SR;Williams,DS;Ho,C
通讯作者：
Ho,C