Intracellular imaging of host‐pathogen interactions using combined CARS and two‐photon fluorescence microscopies
Intracellular imaging of host‐pathogen interactions using combined CARS and two‐photon fluorescence microscopies
复制标题
使用 CARS 和双光子荧光显微镜相结合的宿主-病原体相互作用的细胞内成像
DOI：
--
复制
发表时间：
2010
影响因子：
2.8
通讯作者：
J. Crain
中科院分区：
文献类型：
--
作者：
I. Robinson;Michael A. Ochsenkühn;C. Campbell;G. Giraud;W. Hossack;J. Arlt;J. Crain
细胞内成像是研究宿主-病原体相互作用的重要工具。这一领域的进展尤其是为了了解病毒感染过程对宿主细胞及其代谢功能的影响，包括那些宿主细胞脂代谢因感染而受到调节的情况。我们展示了结合使用相干反斯托克斯拉曼散射(CARS)和双光子荧光显微镜来成像被巨细胞病毒感染的成纤维细胞。CARS用于对宿主细胞膜、脂滴和细胞核的形态进行成像。在感染过程中发现细胞核扩大，面积大约增加一倍。一些细胞还在核周积聚脂滴。使用表达绿色荧光蛋白的转基因病毒株也能够对相同的细胞进行双光子成像，以揭示病毒蛋白表达的位置、性质和程度。(2010Wiley-VCH Verlag GmbH&Co.KGaA，Weinheim)
Intracellular imaging is a key tool in the investigation of host‐pathogen interactions. Advances in this area are particularly sought to understand the effect of viral infection processes on the host cell and its metabolic functions including those cases where host cell lipid metabolism is modulated as a result of infection. We demonstrate the use of combined coherent anti‐Stokes Raman scattering (CARS) and two‐photon fluorescence microscopies to image fibroblast cells infected by cytomegalovirus. CARS is used to image the host cell membrane, lipid droplets and morphology of the nucleus. Cell nuclei are found to expand during infection, approximately doubling in area. Some cells also show accumulations of lipid droplets at the nuclear periphery. Using a genetically modified virus strain expressing the green fluorescent protein also enables two‐photon imaging of the same cells to reveal the location, nature and extent of viral protein expression. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
影响因子：
3.8
作者：
Chen H;Wang H;Slipchenko MN;Jung Y;Shi Y;Zhu J;Buhman KK;Cheng JX
通讯作者：
Cheng JX
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW