A multimodal platform for nonlinear optical microscopy and microspectroscopy.
A multimodal platform for nonlinear optical microscopy and microspectroscopy.
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DOI:
10.1364/oe.17.001282
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发表时间:
2009-02-02
期刊:
影响因子:
3.8
通讯作者:
Cheng JX
中科院分区:
文献类型:
--
作者:
Chen H;Wang H;Slipchenko MN;Jung Y;Shi Y;Zhu J;Buhman KK;Cheng JX
Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.
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