Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.
Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.
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DOI：
10.1371/journal.pone.0100572
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cossins A
中科院分区：
文献类型：
--
作者：
Wilson R;Bourne C;Chaudhuri RR;Gregory R;Kenny J;Cossins A
如果能够通过较少轮次的选择和扩增获得核酸适体，那么核酸适体的鉴定将会取得进展。在本文中，使用下一代测序和基序寻找信息学对 SELEX 和单步选择对凝血酶二价适体的鉴定进行了比较。结果表明，两种方法都鉴定出相似的适体。这很重要，因为它表明下一代测序和基序寻找信息学有可能通过避免多轮酶促转录和扩增来简化适体的选择。
The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potential to simplify the selection of aptamers by avoiding multiple rounds of enzymatic transcription and amplification.
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影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
DOI：
10.3390/s8021090
发表时间：
2008-02-19
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hasegawa H;Taira KI;Sode K;Ikebukuro K
通讯作者：
Ikebukuro K
影响因子：
14.9
作者：
Kuwahara M;Nagashima J;Hasegawa M;Tamura T;Kitagata R;Hanawa K;Hososhima S;Kasamatsu T;Ozaki H;Sawai H
通讯作者：
Sawai H
DOI：
10.1073/pnas.1009331107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Cho, Minseon;Xiao, Yi;Soh, H. Tom
通讯作者：
Soh, H. Tom
影响因子：
14.9
作者：
Ahmad KM;Xiao Y;Soh HT
通讯作者：
Soh HT