Diversity and Hidden Host Specificity of Chytrids Infecting Colonial Volvocacean Algae
Diversity and Hidden Host Specificity of Chytrids Infecting Colonial Volvocacean Algae
复制标题
感染群生藻类的壶菌的多样性和隐藏宿主特异性
DOI：
10.1111/jeu.12632
复制
发表时间：
2018
影响因子：
2.2
通讯作者：
Grossart
中科院分区：
文献类型：
--
作者：
Van den Wyngaert;Rojas-Jimenez;Kagami;Grossart
壶菌是游动孢子真菌，在水生生态系统中发挥着重要但尚未得到充分研究的生态作用。许多壶菌物种在形态学上被描述为浮游植物上的寄生虫。然而，其中大多数很少被分离出来，并且缺乏 DNA 序列数据。在这项研究中，我们分离并培养了三种寄生壶菌，它们感染一种常见的Volvocean宿主物种Yamagishiella unicocca。为了鉴定壶菌，我们对形态和生命周期进行了表征，并基于 18S 和 28S rDNA 基因分析了系统发育关系。壶菌的宿主范围和特异性通过宿主菌株的交叉感染测定来确定，以 rbcL 和 ITS 标记为特征。我们能够确认两种壶菌菌株的身份，即 Endocoenobium eudorinaeIngold 和 Dangeardia mamillataSchröder，并将第三种壶菌菌株描述为 Algomyces stechlinensisgen。等十一月这三种壶菌被分配到壶菌门内的新的、系统发育上较远的分支，每个分支都表现出不同的宿主特异性。通过整合寄生壶菌及其各自宿主物种的形态和分子数据，我们揭示了神秘的宿主-寄生虫关联。这项研究强调，（伪）隐性多样性的高盛行需要对浮游植物宿主和寄生壶菌进行分子表征，以准确识别和比较宿主范围和特异性，并研究浮游植物与壶菌的总体相互作用。
Chytrids are zoosporic fungi that play an important, but yet understudied, ecological role in aquatic ecosystems. Many chytrid species have been morphologically described as parasites on phytoplankton. However, the majority of them have rarely been isolated and lack DNA sequence data. In this study we isolated and cultivated three parasitic chytrids, infecting a common volvocacean host species,Yamagishiella unicocca. To identify the chytrids, we characterized morphology and life cycle, and analyzed phylogenetic relationships based on 18S and 28S rDNA genes. Host range and specificity of the chytrids was determined by cross‐infection assays with host strains, characterized byrbcL and ITS markers. We were able to confirm the identity of two chytrid strains asEndocoenobium eudorinaeIngold andDangeardia mamillataSchröder and described the third chytrid strain asAlgomyces stechlinensisgen. et sp. nov. The three chytrids were assigned to novel and phylogenetically distant clades within the phylum Chytridiomycota, each exhibiting different host specificities. By integrating morphological and molecular data of both the parasitic chytrids and their respective host species, we unveiled cryptic host‐parasite associations. This study highlights that a high prevalence of (pseudo)cryptic diversity requires molecular characterization of both phytoplankton host and parasitic chytrid to accurately identify and compare host range and specificity, and to study phytoplankton‐chytrid interactions in general.
登录
查看更多内容
影响因子：
2.2
作者：
K. Seto;M. Kagami;Yousuke Degawa
通讯作者：
K. Seto;M. Kagami;Yousuke Degawa
影响因子：
--
作者：
Mozley-Standridge, Sharon E.;Letcher, Peter M.;Simmons, D. Rabern
通讯作者：
Simmons, D. Rabern
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
淡水浮游硅藻 Asterionella formosa 中隐藏的多样性
影响因子：
4.9
作者：
S. Wyngaert;S. Wyngaert;S. Wyngaert;M. Möst;M. Möst;M. Möst;Remo Freimann;B. Ibelings;B. Ibelings;B. Ibelings;P. Spaak;P. Spaak
通讯作者：
P. Spaak
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro