RNA-seq analysis reveals the role of red light in resistance against Pseudomonas syringae pv. tomato DC3000 in tomato plants.
RNA-seq analysis reveals the role of red light in resistance against Pseudomonas syringae pv. tomato DC3000 in tomato plants.
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RNA-seq 分析揭示了红光在抵抗丁香假单胞菌 pv 的作用。
DOI：
10.1186/s12864-015-1228-7
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发表时间：
2015-02-25
期刊：
影响因子：
4.4
通讯作者：
Zhou YH
中科院分区：
文献类型：
--
作者：
Yang YX;Wang MM;Yin YL;Onac E;Zhou GF;Peng S;Xia XJ;Shi K;Yu JQ;Zhou YH
背景植物减弱了它们对各种细菌和真菌病原体的反应，导致病原体在夜间感染的发生率较高。然而，鲜为人知的是负责光诱导的防御反应的分子机制;转录组数据可能会促进阐明这种mechanism.ResultsIn这项研究中，我们观察到番茄耐假单胞菌pv.番茄DC 3000（PtoDC 3000），午夜前感病最强。夜间光照处理，特别是红光处理，显着提高了抗性，这种效果与水杨酸（SA）的积累和防御相关基因的转录增加。RNA-seq分析显示，红光诱导了一组与光敏色素和SA调节的抗性反应有关的昼夜节律相关基因。Pto DC 3000感染和红光共同调控多种植物激素（生长素、SA、茉莉酸和乙烯）的生物合成和信号传导途径，其中SA途径受红光和Pto DC 3000感染的影响最为显著。这一结果表明SA介导的信号通路参与红光诱导的对病原体的抗性。重要的是，病程相关基因1（NPR 1）的非表达沉默部分损害了红光诱导的抗Pto DC 3000的抗性。此外，参与氧化还原稳态的基因组（呼吸爆发氧化酶同源物，RBOH;谷胱甘肽S-转移酶，GST;糖基转移酶，GT），钙（钙调素，CAM;钙调素结合蛋白，CBP），和防御（多酚氧化酶，PPO; NUDX 1）以及转录因子（WRKY 18，WRKY 53，WRKY 60，WRKY 70）和纤维素合成酶在转录水平上被红光诱导，以响应病原菌的攻击。我们的结果表明，有一个昼夜变化的敏感性Pto DC 3000与最大的敏感性在晚上。红光诱导的抗Pto DC 3000在夜间与增强SA途径，纤维素合成酶，和减少氧化还原稳态。
BackgroundPlants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilitate the elucidation of this mechanism.ResultsIn this study, we observed diurnal changes in tomato resistance to Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), with the greatest susceptibility before midnight. Nightly light treatment, particularly red light treatment, significantly enhanced the resistance; this effect was correlated with increased salicylic acid (SA) accumulation and defence-related gene transcription. RNA-seq analysis revealed that red light induced a set of circadian rhythm-related genes involved in the phytochrome and SA-regulated resistance response. The biosynthesis and signalling pathways of multiple plant hormones (auxin, SA, jasmonate, and ethylene) were co-ordinately regulated following Pto DC3000 infection and red light, and the SA pathway was most significantly affected by red light and Pto DC3000 infection. This result indicates that SA-mediated signalling pathways are involved in red light-induced resistance to pathogens. Importantly, silencing of nonexpressor of pathogensis-related genes 1 (NPR1) partially compromised red light-induced resistance against Pto DC3000. Furthermore, sets of genes involved in redox homeostasis (respiratory burst oxidase homologue, RBOH; glutathione S-transferases, GSTs; glycosyltransferase, GTs), calcium (calmodulin, CAM; calmodulin-binding protein, CBP), and defence (polyphenol oxidase, PPO; nudix hydrolase1, NUDX1) as well as transcription factors (WRKY18, WRKY53, WRKY60, WRKY70) and cellulose synthase were differentially induced at the transcriptional level by red light in response to pathogen challenge.ConclusionsTaken together, our results suggest that there is a diurnal change in susceptibility to Pto DC3000 with greatest susceptibility in the evening. The red light induced-resistance to Pto DC3000 at night is associated with enhancement of the SA pathway, cellulose synthase, and reduced redox homeostasis.
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