Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock.
Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock.
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DOI：
10.1371/journal.pone.0026968
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Roden LC
中科院分区：
文献类型：
--
作者：
Bhardwaj V;Meier S;Petersen LN;Ingle RA;Roden LC
生物钟允许植物预测非生物刺激（如光）的可预测的每日变化;然而，生物钟是否同样允许植物预测与其他生物体的相互作用尚不清楚。在这里，我们表明，拟南芥（拟南芥）有昼夜节律钟介导的变化，在抗性的毒性细菌病原体假单胞菌pv。番茄DC 3000（Pst DC 3000），其中植物在主观早晨对感染最不敏感。我们认为，增加的抗Pst DC 3000观察上午在Col-0植物的结果从时钟介导的调制病原体相关的分子模式（PAMP）触发的免疫。公开可用的微阵列数据的分析显示，大量的拟南芥防御相关的基因表现出昼夜和昼夜调节，包括参与感知的PAMP鞭毛蛋白，表现出在早上的表达高峰的基因。因此，我们观察到，PAMP触发的胼胝质沉积是显着较高的野生型植物接种Pst DC 3000 hrpA在主观上午比晚上，而没有这样的时间差异是明显的，在pastemic植物。我们的研究结果表明，PAMP引发的免疫反应是由昼夜节律钟调节的，时间调节使植物能够在白天更有效地预测和应对病原体的挑战。
The circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest that the increased resistance to Pst DC3000 observed in the morning in Col-0 plants results from clock-mediated modulation of pathogen associated molecular pattern (PAMP)-triggered immunity. Analysis of publicly available microarray data revealed that a large number of Arabidopsis defence-related genes showed both diurnal- and circadian-regulation, including genes involved in the perception of the PAMP flagellin which exhibit a peak in expression in the morning. Accordingly, we observed that PAMP-triggered callose deposition was significantly higher in wild-type plants inoculated with Pst DC3000 hrpA in the subjective morning than in the evening, while no such temporal difference was evident in arrhythmic plants. Our results suggest that PAMP-triggered immune responses are modulated by the circadian clock and that temporal regulation allows plants to anticipate and respond more effectively to pathogen challenges in the daytime.
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影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
影响因子：
7.4
作者：
Fowler, SG;Cook, D;Thomashow, ME
通讯作者：
Thomashow, ME
DOI：
10.1073/pnas.0600666103
发表时间：
2006-05-23
影响因子：
11.1
作者：
Chen, Zhi;Laurence, Arian;O'Shea, John J.
通讯作者：
O'Shea, John J.
影响因子：
12.3
作者：
Covington, Michael F.;Maloof, Julin N.;Straume, Marty;Kay, Steve A.;Harmer, Stacey L.
通讯作者：
Harmer, Stacey L.
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J