Changes in expression of bcl-2 and bax in Syrian hamster embryo (SHE) cells exposed to ZnCl2
Changes in expression of bcl-2 and bax in Syrian hamster embryo (SHE) cells exposed to ZnCl2
复制标题
暴露于 ZnCl2 的叙利亚仓鼠胚胎 (SHE) 细胞中 bcl-2 和 bax 表达的变化
作者：
M. Maire;C. Rast;C. Pagnout;P. Vasseur
锌参与许多生理过程，在功能细胞和结构细胞中起着关键作用。100 ~ 150 μmol L−1浓度的锌可以诱导叙利亚仓鼠胚胎（SHE）细胞的形态转变。在这些浓度下，锌可以抑制SHE细胞的凋亡。本研究的目的是阐明锌在细胞凋亡通路中的作用机制。采用RT-PCR方法研究了100 μmol L−1 ZnCl2和150 μmol L−1 ZnCl2对SHE细胞Bcl-2家族成员蛋白表达和转录因子c-Myc表达的影响。未观察到对原癌基因c-myc的影响。bcl-2表达上调，bax表达降低。免疫印迹证实了这些变化。在喜树碱处理的凋亡SHE细胞中，Zn2+对bcl-2/bax比值的影响得到证实。从RT-PCR扩增中获得的克隆和测序的cdna使我们能够检查RT-PCR产物编码预期的蛋白质。本研究表明，锌通过改变正常和凋亡SHE细胞的bcl-2/bax比值，在凋亡过程的早期阶段起作用。
Zinc is involved in many physiological processes and plays a critical role in functional and structural cells. Zinc at concentrations ranging from 100 to 150 μmol L−1 has been shown to induce morphological transformation of Syrian hamster embryo (SHE) cells. At these concentrations, zinc inhibited apoptosis in SHE cells. The objective of this study was to elucidate the mechanisms of action of zinc on the apoptotic pathway. Effects of 100 and 150 μmol L−1 ZnCl2 on the expression of two members of the Bcl-2 family of proteins and on the transcription factor c-Myc in SHE cells was investigated using RT-PCR. No effect on the proto-oncogene c-myc was observed. Up-regulation of bcl-2 expression was found and bax expression was reduced. These changes have been corroborated by immunoblotting. Effects of Zn2+ on bcl-2/bax ratio were confirmed in apoptotic camptothecin-treated SHE cells. Cloned and sequenced cDNAs obtained from RT-PCR amplifications allowed us to check the RT-PCR products encoded the expected proteins. This study demonstrated that zinc acts in the early phases of the apoptotic process by modification of the bcl-2/bax ratio in normal and apoptotic SHE cells.
DOI：
10.1006/bbrc.2000.2608
发表时间：
2000-04-29
影响因子：
3.1
作者：
Jankowski-Hennig, MA;Clegg, MS;Keen, CL
通讯作者：
Keen, CL
叙利亚仓鼠胚胎细胞转化与模型和编码化学物质的实验室间比较。
DOI：
10.1002/em.2860080108
发表时间：
1986
期刊：
Environmental mutagenesis
影响因子：
--
作者：
Tu,A;Hallowell,W;Pallotta,S;Sivak,A;Lubet,RA;Curren,RD;Avery,MD;Jones,C;Sedita,BA;Huberman,E
通讯作者：
Huberman,E