MECOM/PRDM3 and PRDM16 Serve as Prognostic-Related Biomarkers and Are Correlated With Immune Cell Infiltration in Lung Adenocarcinoma.
MECOM/PRDM3 and PRDM16 Serve as Prognostic-Related Biomarkers and Are Correlated With Immune Cell Infiltration in Lung Adenocarcinoma.
复制标题
MECOM/PRDM3 和 PRDM16 作为预后相关生物标志物，与肺腺癌中的免疫细胞浸润相关
DOI：
10.3389/fonc.2022.772686
复制
发表时间：
2022
影响因子：
4.7
通讯作者：
Shi P
中科院分区：
文献类型：
--
作者：
Li M;Ren H;Zhang Y;Liu N;Fan M;Wang K;Yang T;Chen M;Shi P
MDS1和EVI1复合体位点（MECOM，也称为PRDM3）和PR结构域16 （PRDM16）是两个高度相关的锌指转录因子，与许多恶性肿瘤相关。然而，MECOM和PRDM16在肺腺癌（LUAD）预后和肿瘤免疫浸润中的作用机制尚不明确。检索肿瘤基因组图谱（Cancer Genome Atlas， TCGA）、Oncomine、UALCAN、GEPIA和TIMER数据库，以确定LUAD中MECOM和PRDM16的表达与临床病理特征、免疫浸润和预后的关系。通过cbiopportal研究了这两个基因的共表达基因，并通过GO和KEGG分析阐明了MECOM-和prdm16相关基因的潜在机制。利用STRING数据库进一步构建共表达基因的蛋白-蛋白相互作用网络，并利用Cytoscape对中心基因进行鉴定。最后，采用qRT-PCR方法鉴定LUAD中靶基因的mRNA水平。MECOM和PRDM16 mRNA水平在LUAD中下调（p < 0.05），且这两个基因的低表达与LUAD患者的年龄、性别、吸烟时间、组织亚型、不良分期、淋巴结转移情况、TP53突变及预后相关（p < 0.05）。MECOM和PRDM16也被发现与多种免疫细胞亚群及其标记物的表达相关。KEGG分析显示，两者主要富集于细胞周期、细胞衰老、DNA复制和p53信号通路。重要的是，通过qRT-PCR也发现LUAD临床样本中这两个基因的mRNA水平降低。MECOM和PRDM16可能作为控制免疫细胞向LUAD募集的潜在预后生物标志物。
The MDS1 and EVI1 complex locus (MECOM, also called PRDM3) and PR domain containing 16 (PRDM16) are two highly related zinc finger transcription factors associated with many malignancies. However, the mechanisms of MECOM and PRDM16 in prognosis and tumor immune infiltration in lung adenocarcinoma (LUAD) remain uncertain. The Cancer Genome Atlas (TCGA), Oncomine, UALCAN, GEPIA, and TIMER databases were searched to determine the relationship between the expression of MECOM and PRDM16, clinicopathological features, immune infiltration, and prognosis in LUAD. Coexpressed genes of the two genes were investigated by CBioPortal, and the potential mechanism of MECOM- and PRDM16-related genes was elucidated by GO and KEGG analyses. STRING database was utilized to further construct the protein-protein interaction network of the coexpressed genes, and the hub genes were identified by Cytoscape. Finally, qRT-PCR was performed to identify the mRNA levels of the target genes in LUAD. mRNA levels of MECOM and PRDM16 were downregulated in LUAD (p < 0.05), and the low expression of the two genes was associated with the age, gender, smoking duration, tissue subtype, poor stage, nodal metastasis status, TP53 mutation, and prognosis in LUAD (p < 0.05). MECOM and PRDM16 were also found to be correlated with the expression of a variety of immune cell subsets and their markers. KEGG analysis showed that both of them were mainly enriched in the cell cycle, cellular senescence, DNA replication, and p53 signaling pathway. Importantly, the mRNA levels of the two genes were also found to be decreased in the clinical samples of LUAD by qRT-PCR. MECOM and PRDM16 may serve as potential prognostic biomarkers which govern immune cell recruitment to LUAD.
登录
查看更多内容
影响因子：
28.2
作者：
Cai SF;Chu SH;Goldberg AD;Parvin S;Koche RP;Glass JL;Stein EM;Tallman MS;Sen F;Famulare CA;Cusan M;Huang CH;Chen CW;Zou L;Cordner KB;DelGaudio NL;Durani V;Kini M;Rex M;Tian HS;Zuber J;Baslan T;Lowe SW;Rienhoff HY Jr;Letai A;Levine RL;Armstrong SA
通讯作者：
Armstrong SA
影响因子：
5.2
作者：
Chi A;He X;Hou L;Nguyen NP;Zhu G;Cameron RB;Lee JM
通讯作者：
Lee JM
影响因子：
20.3
作者：
Mochizuki, N;Shimizu, S;Morishita, K
通讯作者：
Morishita, K
影响因子：
14.9
作者：
Ivanochko, Danton;Halabelian, Levon;Arrowsmith, Cheryl H.
通讯作者：
Arrowsmith, Cheryl H.
影响因子：
8
作者：
Mitani, K
通讯作者：
Mitani, K