The scaffolding protein SYD-2/Liprin-α regulates the mobility and polarized distribution of dense-core vesicles in C. elegans motor neurons.
The scaffolding protein SYD-2/Liprin-α regulates the mobility and polarized distribution of dense-core vesicles in C. elegans motor neurons.
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DOI：
10.1371/journal.pone.0054763
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Juo P
中科院分区：
文献类型：
--
作者：
Goodwin PR;Juo P
轴突和树突成分的极化运输对于神经元结构和功能的发育和维持至关重要。含有神经肽的致密核心（DCV）囊泡以极化的方式从细胞体运输到其释放位点;然而，参与这一过程的分子并不明确。在这里，我们表明支架蛋白SYD-2/Liprin-α是需要正常的极化定位静脉标记的神经肽的胆碱能运动神经元轴突在C。优雅在syd-2功能丧失突变体中，含有INS-22神经肽的DCV在运动神经元中的正常极化定位被破坏，DCV在细胞体和树突中积累。移动的DCV的延时显微镜和记波分析显示，syd-2突变体表现出在顺行和逆行方向上移动的DCV数量减少，并且在轴突连合和树突中静止的DCV相应增加。此外，DCV运行长度和速度下降，在轴突连合和树突的syd-2突变体。这项研究表明，SYD-2促进DCV的双向流动性，并确定SYD-2作为DCV运输和极化分布的新调节剂。
The polarized trafficking of axonal and dendritic components is essential for the development and maintenance of neuronal structure and function. Neuropeptide-containing dense-core (DCVs) vesicles are trafficked in a polarized manner from the cell body to their sites of release; however, the molecules involved in this process are not well defined. Here we show that the scaffolding protein SYD-2/Liprin-α is required for the normal polarized localization of Venus-tagged neuropeptides to axons of cholinergic motor neurons in C. elegans. In syd-2 loss of function mutants, the normal polarized localization of INS-22 neuropeptide-containing DCVs in motor neurons is disrupted, and DCVs accumulate in the cell body and dendrites. Time-lapse microscopy and kymograph analysis of mobile DCVs revealed that syd-2 mutants exhibit decreased numbers of DCVs moving in both anterograde and retrograde directions, and a corresponding increase in stationary DCVs in both axon commissures and dendrites. In addition, DCV run lengths and velocities were decreased in both axon commissures and dendrites of syd-2 mutants. This study shows that SYD-2 promotes bi-directional mobility of DCVs and identifies SYD-2 as a novel regulator of DCV trafficking and polarized distribution.
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分析突触蛋白鉴定了胰岛素分泌和寿命的调节剂。
影响因子：
4.5
作者：
Ch'ng, QueeLim;Sieburth, Derek;Kaplan, Joshua M.
通讯作者：
Kaplan, Joshua M.
影响因子：
16.2
作者：
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影响因子：
7.5
作者：
Borgonovo, Barbara;Ouwendijk, Joke;Solimena, Michele
通讯作者：
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影响因子：
3.3
作者：
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通讯作者：
Saxton, William M.
DOI：
10.1523/jneurosci.1862-10.2010
发表时间：
2010-11-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Astigarraga S;Hofmeyer K;Farajian R;Treisman JE
通讯作者：
Treisman JE