Functional characterization of an allatotropin receptor expressed in the corpora allata of mosquitoes.
Functional characterization of an allatotropin receptor expressed in the corpora allata of mosquitoes.
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DOI：
10.1016/j.peptides.2011.07.025
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发表时间：
2012-03
期刊：
影响因子：
3
通讯作者：
Noriega FG
中科院分区：
文献类型：
--
作者：
Nouzova M;Brockhoff A;Mayoral JG;Goodwin M;Meyerhof W;Noriega FG
Allatotropin是一种昆虫神经肽，对多种不同组织具有多效性作用。在目前的工作中，我们描述了埃及伊蚊allatotropin受体（AeATr）的鉴定，克隆和功能和分子特征，并提供了详细的定量研究AeATr基因在成年蚊子的表达。对成年雌性AeATr mRNA的组织分布的分析显示，在神经系统（脑、腹、胸和腹神经节）、咽侧体-心侧体复合体和卵巢中有较高的转录水平。该受体也在心脏、后肠、雄性睾丸和附属腺中表达。分离咽侧体（CA）和心侧体，然后分析分离的腺体中的基因表达，结果显示AeATr主要在CA中表达。在雌性CA中，AeATr mRNA水平在蛹早期较低，在成虫羽化前6小时开始增加，在雌性羽化后24小时达到最大值。血液喂养导致转录水平下降。AeATr mRNA的变化模式类似于JH生物合成的变化。在表达AeATr的HEK 293细胞中钙瞬变的荧光成像读板器记录显示对A.在低纳摩尔浓度范围内的埃及异生长素刺激。我们的研究表明，AeATr在蚊子JH合成的调节中发挥作用。
Allatotropin is an insect neuropeptide with pleiotropic actions on a variety of different tissues. In the present work we describe the identification, cloning and functional and molecular characterization of an Aedes aegypti allatotropin receptor (AeATr) and provide a detailed quantitative study of the expression of the AeATr gene in the adult mosquito. Analysis of the tissue distribution of AeATr mRNA in adult female revealed high transcript levels in the nervous system (brain, abdominal, thoracic and ventral ganglia), corpora allata-corpora cardiaca complex and ovary. The receptor is also expressed in heart, hindgut and male testis and accessory glands. Separation of the corpora allata (CA) and corpora cardiaca followed by analysis of gene expression in the isolated glands revealed expression of the AeATr primarily in the CA. In the female CA, the AeATr mRNA levels were low in the early pupae, started increasing 6 hours before adult eclosion and reached a maximum 24 hours after female emergence. Blood feeding resulted in a decrease in transcript levels. The pattern of changes of AeATr mRNA resembles the changes in JH biosynthesis. Fluorometric Imaging Plate Reader recordings of calcium transients in HEK293 cells expressing the AeATr showed a selective response to A. aegypti allatotropin stimulation in the low nanomolar concentration range. Our studies suggest that the AeATr play a role in the regulation of JH synthesis in mosquitoes.
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影响因子：
30.8
作者：
Bufe, B;Hofmann, T;Meyerhof, W
通讯作者：
Meyerhof, W
影响因子：
--
作者：
DAVIS, D;LIU, XB;SEGALOFF, DL
通讯作者：
SEGALOFF, DL
影响因子：
6.1
作者：
Behrens, Maik;Brockhoff, Anne;Meyerhof, Wolfgang
通讯作者：
Meyerhof, Wolfgang
影响因子：
4.8
作者：
Kreienkamp, HJ;Larusson, HJ;Richter, D
通讯作者：
Richter, D
影响因子：
2.2
作者：
Hernandez-Martinez, Salvador;Mayoral, Jaime G.;Noriega, Fernando G.
通讯作者：
Noriega, Fernando G.