Identification of the principal promoter sequence of the c-H-ras transforming oncogene: deletion analysis of the 5'-flanking region by focus formation assay
Identification of the principal promoter sequence of the c-H-ras transforming oncogene: deletion analysis of the 5'-flanking region by focus formation assay
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c-H-ras 转化癌基因主要启动子序列的鉴定：通过病灶形成实验对 5 侧翼区域进行缺失分析
DOI：
10.1128/mcb.7.8.2933-2940.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
H. Honkawa;W. Masahashi;S. Hashimoto;T. Hashimoto
分离出许多缺失突变体，包括与哈维肉瘤病毒（c-H-ras）相关的人类细胞癌基因（c-H-ras）的5'、3'和5'侧翼区域的内部缺失，并在NIH 3T3细胞中检查了它们的转化活性。在不丧失转化活性的情况下无法检测到的DNA序列位于该区域上游相对较短的一段区域，该区域与v-H-ras癌基因的5'-侧翼区域具有同源性。 S1核酸酶分析表明，在第一个编码ATG上游约1,371和1,298个碱基对的位置处存在两簇mRNA起始位点。启动子功能所需的最小区域估计为 51 个碱基对长（或更少）的 DNA 片段。该启动子富含 GC (78%)，不包含通常在 PolII 定向启动子中观察到的共有序列，但包含一个 GC 盒，其中包含 mRNA 起始位点之一。此外，两组正元件和负元件似乎位于启动子和蛋白质编码区之间，这似乎分别对c-H-ras癌基因的转化效率产生正向和负向影响。
A number of deletion mutants were isolated, including 5', 3', and internal deletions in the 5'-flanking region of the human cellular oncogene related to the Harvey sarcoma virus (c-H-ras), and their transforming activities were examined in NIH 3T3 cells. DNA sequences which could not be detected without losing transforming activity were localized to a relatively short stretch upstream of the region which showed homology to the 5'-flanking region of v-H-ras oncogene. S1 nuclease analysis indicated that there were two clusters of mRNA start sites at positions that were about 1,371 and 1,298 base pairs upstream of the first coding ATG. The minimum region required for promoter function was estimated to be a 51-base-pair-long (or less) DNA segment. The promoter was GC rich (78%) and did not contain the consensus sequences that are usually observed in PolII-directed promoters but contained a GC box within which one of the mRNA start sites was included. In addition, two sets of positive and negative elements seemed to be located between the promoter and the protein-coding region, which appeared to influence positively and negatively, respectively, the efficiency of transformation with the c-H-ras oncogene.
小鼠二氢叶酸还原酶 mRNA 5 末端的异质性。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McGrogan,M;Simonsen,CC;Smouse,DT;Farnham,PJ;Schimke,RT
通讯作者：
Schimke,RT
次黄嘌呤磷酸核糖转移酶基因的结构、表达和突变。
DOI：
10.1073/pnas.81.7.2147
发表时间：
1984
影响因子：
11.1
作者：
Melton,DW;Konecki,DS;Brennand,J;Caskey,CT
通讯作者：
Caskey,CT
DOI：
10.1073/pnas.82.12.3978
发表时间：
1985-01-01
影响因子：
11.1
作者：
FARNHAM, PJ;ABRAMS, JM;SCHIMKE, RT
通讯作者：
SCHIMKE, RT