In-situ optical and acoustical measurements of the buoyant cyanobacterium p. Rubescens: spatial and temporal distribution patterns.
In-situ optical and acoustical measurements of the buoyant cyanobacterium p. Rubescens: spatial and temporal distribution patterns.
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DOI：
10.1371/journal.pone.0080913
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peeters F
中科院分区：
文献类型：
--
作者：
Hofmann H;Peeters F
测试了光学（荧光）和声学原位技术测量淡水生态系统浮游生物时空分布的能力，特别强调了有害和漂浮的蓝藻P. rubescens。用多光谱FluoroProbe（Moldaenke FluoroProbe，MFP）和Seapoint叶绿素荧光计（SCF）测量荧光。现场测量的声学后向散射强度（ABS）进行了三个不同的声学设备，覆盖多个声频（614 kHz ADCP，2 MHz ADP，和6 MHz ADV）。MFP提供了一种快速可靠的技术来测量不同波长的荧光，从而可以区分P. rubescens和其他浮游植物物种。所有三种声学装置都对P.rubescens敏感，即使其他散射体，浮游动物或悬浮沉积物存在于水柱中，因为含有气泡的P.rubescens具有很强的密度差，因此与环境水和其他散射体形成声学对比。经过校准后，光学和声学测量的组合不仅可以定性和定量观察P.rubescens，而且可以区分P.rubescens与其他浮游植物和浮游动物。由于测量装置可以在原地高速采样，因此能够以高时间（分钟）和空间（分米）分辨率或覆盖大的时间（季节）和空间（流域尺度）尺度评估浮游生物分布。
Optical (fluorescence) and acoustic in-situ techniques were tested in their ability to measure the spatial and temporal distribution of plankton in freshwater ecosystems with special emphasis on the harmful and buoyant cyanobacterium P. rubescens. Fluorescence was measured with the multi-spectral FluoroProbe (Moldaenke FluoroProbe, MFP) and a Seapoint Chlorophyll Fluorometer (SCF). In-situ measurements of the acoustic backscatter strength (ABS) were conducted with three different acoustic devices covering multiple acoustic frequencies (614 kHz ADCP, 2 MHz ADP, and 6 MHz ADV). The MFP provides a fast and reliable technique to measure fluorescence at different wavelengths in situ, which allows discriminating between P. rubescens and other phytoplankton species. All three acoustic devices are sensitive to P. rubescens even if other scatterers, e.g., zooplankton or suspended sediment, are present in the water column, because P. rubescens containing gas vesicles has a strong density difference and hence acoustic contrast to the ambient water and other scatterers. After calibration, the combination of optical and acoustical measurements not only allows qualitative and quantitative observation of P. rubescens, but also distinction between P. rubescens, other phytoplankton, and zooplankton. As the measuring devices can sample in situ at high rates they enable assessment of plankton distributions at high temporal (minutes) and spatial (decimeters) resolution or covering large temporal (seasonal) and spatial (basin scale) scales.
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