A Trabecular Bone Explant Model of Osteocyte-Osteoblast Co-Culture for Bone Mechanobiology.
A Trabecular Bone Explant Model of Osteocyte-Osteoblast Co-Culture for Bone Mechanobiology.
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DOI：
10.1007/s12195-009-0075-5
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发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Guo, X. Edward
中科院分区：
文献类型：
--
作者：
Chan, Meilin Ete;Lu, Xin L.;Huo, Bo;Baik, Andrew D.;Chiang, Victor;Guldberg, Robert E.;Lu, Helen H.;Guo, X. Edward
骨细胞网络被认为是骨重建过程中的主要力学传感器，而骨细胞-成骨细胞通讯在协调骨形成和骨转换过程中起着重要的中介作用。在这项研究中，我们开发了一种新的3D骨小梁共培养模型，允许位于天然细胞外基质环境中的活骨细胞与骨表面种植的成骨细胞相互连接。使用低水平的介质灌流系统，骨组织中原位骨细胞的活性可维持长达4周，并成功地在骨细胞和种植的原代成骨细胞之间建立了功能性缝隙连接细胞间通讯(GJIC)。利用这种新的共培养模型，进一步研究了动态变形负荷、GJIC和前列腺素E2(PGE2)释放对功能性骨适应的影响。结果表明，动态变形载荷能显著增加骨细胞释放前列腺素E_2，促进骨形成，提高骨移植材料的表观弹性模数。然而，缝隙连接或PGE2通路的抑制显著减弱了机械负荷的影响。这种三维松质骨外植体共培养模型有很大的潜力来填补关于骨细胞作为力学传感器的作用以及骨细胞如何传递信号来调节成骨细胞的功能和反映在其力学特性中的骨骼完整性的知识空白。
The osteocyte network is recognized as the major mechanical sensor in the bone remodeling process, and osteocyte–osteoblast communication acts as an important mediator in the coordination of bone formation and turnover. In this study, we developed a novel 3D trabecular bone explant co-culture model that allows live osteocytes situated in their native extracellular matrix environment to be interconnected with seeded osteoblasts on the bone surface. Using a low-level medium perfusion system, the viability of in situ osteocytes in bone explants was maintained for up to 4 weeks, and functional gap junction intercellular communication (GJIC) was successfully established between osteocytes and seeded primary osteoblasts. Using this novel co-culture model, the effects of dynamic deformational loading, GJIC, and prostaglandin E2 (PGE2) release on functional bone adaptation were further investigated. The results showed that dynamical deformational loading can significantly increase the PGE2 release by bone cells, bone formation, and the apparent elastic modulus of bone explants. However, the inhibition of gap junctions or the PGE2 pathway dramatically attenuated the effects of mechanical loading. This 3D trabecular bone explant co-culture model has great potential to fill in the critical gap in knowledge regarding the role of osteocytes as a mechano-sensor and how osteocytes transmit signals to regulate osteoblasts function and skeletal integrity as reflected in its mechanical properties.
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影响因子：
3.3
作者：
Cherian, PP;Siller-Jackson, AJ;Jiang, JX
通讯作者：
Jiang, JX
影响因子：
4.1
作者：
Alford, AI;Jacobs, CR;Donahue, HJ
通讯作者：
Donahue, HJ
DOI：
10.1073/pnas.202296599
发表时间：
2002-10-01
影响因子：
11.1
作者：
Bancroft, GN;Sikavitsast, VI;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
--
作者：
Cartmell, SH;Porter, BD;Guldberg, RE
通讯作者：
Guldberg, RE
影响因子：
2.4
作者：
BURGER, EH;KLEINNULEND, J;VELDHUIJZEN, JP
通讯作者：
VELDHUIJZEN, JP