Glucocorticoids reduce intracellular calcium concentration and protects neurons against glutamate toxicity.
Glucocorticoids reduce intracellular calcium concentration and protects neurons against glutamate toxicity.
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DOI：
10.1016/j.ceca.2012.12.006
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发表时间：
2013-04
期刊：
影响因子：
4
通讯作者：
Abramov AY
中科院分区：
文献类型：
--
作者：
Suwanjang W;Holmström KM;Chetsawang B;Abramov AY
糖皮质激素是通过糖皮质激素受体起作用的类固醇激素。它们调节各种各样的生物过程。两种糖皮质激素，天然存在的皮质酮和化学产生的地塞米松，已被用来研究皮质共培养的神经元和星形胶质细胞中糖皮质激素对Ca2+信号传导的影响。地塞米松和皮质酮在较小程度上都诱导神经元和星形胶质细胞胞浆Ca2+浓度的降低。这两种化合物的作用可以通过抑制质膜ATP酶、钙调蛋白和应用糖皮质激素受体拮抗剂来阻断，而抑制NMDA受体或内质网钙泵则没有作用。糖皮质激素治疗通过调节响应谷氨酸毒性的延迟钙失调进一步保护免受有害的钙信号传导和细胞死亡。在使用的浓度下，地塞米松和皮质酮本身没有显示出细胞毒性。因此，这些结果表明地塞米松和皮质酮可用于保护细胞免受钙超载。
Glucocorticoids are steroid hormones which act through the glucocorticoid receptor. They regulate a wide variety of biological processes. Two glucocorticoids, the naturally occurring corticosterone and chemically produced dexamethasone, have been used to investigate the effect of glucocorticoids on Ca2+-signalling in cortical co-cultures of neurons and astrocytes. Dexamethasone and to a lesser degree corticosterone both induced a decrease in cytosolic Ca2+ concentration in neurons and astrocytes. The effect of both compounds can be blocked by inhibition of the plasmamembrane ATPase, calmodulin and by application of a glucocorticoid receptor antagonist, while inhibition of NMDA receptors or the endoplasmic reticulum calcium pump had no effect. Glucocorticoid treatment further protects against detrimental calcium signalling and cell death by modulating the delayed calcium deregulation in response to glutamate toxicity. At the concentrations used dexamethasone and corticosterone did not show cell toxicity of their own. Thus, these results indicate that dexamethasone and corticosterone might be used for protection of the cells from calcium overload.
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DOI：
10.1016/j.biocel.2011.03.003
发表时间：
2011-06-01
影响因子：
4
作者：
Domijan, Ana-Marija;Abramov, Andrey Y.
通讯作者：
Abramov, Andrey Y.
影响因子：
4
作者：
Menconi, Michael;Gonnella, Patricia;Petkova, Victoria;Lecker, Stewart;Hasselgren, Per-Olof
通讯作者：
Hasselgren, Per-Olof
影响因子：
2.7
作者：
Kim, Man-Hee;Lee, Geun-Shik;Jeung, Eui-Bae
通讯作者：
Jeung, Eui-Bae
影响因子：
2.3
作者：
Sukocheva, OA;Abramov, AY;Carpenter, DO
通讯作者：
Carpenter, DO
影响因子：
3.4
作者：
Young, AH;Sahakian, BJ;Cowen, PJ
通讯作者：
Cowen, PJ