Unique phenotype of the metamorphosis-defective mutant Ishigameyoh (<i>gap</i>): Establishment of a PCR-based marker for efficacious mutant maintenance in <i>Bombyx mori</i>
Unique phenotype of the metamorphosis-defective mutant Ishigameyoh (<i>gap</i>): Establishment of a PCR-based marker for efficacious mutant maintenance in <i>Bombyx mori</i>
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变态缺陷突变体 Ishigameyoh (<i>gap</i>) 的独特表型：建立基于 PCR 的标记，以有效维持家蚕突变体</i>
DOI：
10.11416/jibs.86.3_095
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发表时间：
2017
影响因子：
--
通讯作者：
Yutaka Banno
中科院分区：
文献类型：
--
作者：
Tsuguru Fujii;Yutaka Banno
Ishigameyoh（gap）是家蚕的一个自发突变体，具有无翅和不育的表型。表型观察显示，差距突变影响成虫盘和成虫器官原基的发育，包括翅膀、腿、复眼和生殖器官。因此，Ishigameyoh（gap）是一种变态缺陷型突变体。我们发现卵子发生依赖于差距基因，而精子发生是独立的。卵巢移植实验表明，在卵子发生过程中对差距基因的细胞自主需求。差距突变通过杂交+缺口/缺口蛾作为u90菌株而得以保持。然而，u90菌株难以维持，因为+ gap/+ gap和+ gap/gap蛾不能在表型上区分。为了有效的维持，我们建立了一个与差距基因座紧密连锁的分子标记。首先，我们使用表型标记将差距位点定位于5号染色体。其次，使用基于PCR的标记将差距基因座缩小到1 Mb区域内。在这个1-Mb区域内，我们最终建立了一个基于PCR的标记，该标记扩增来自u90菌株的+ gap/+ gap和gap/gap后代的不同大小的产物。该标记允许以< 1%的错误率区分+ gap/+ gap和+ gap/gap蛾，因此减少了维持u90菌株所需的劳动。此外，该标记有助于在化蛹前识别+ gap和gap个体，这对于基于幼虫的差距基因的遗传和生理分析是必要的。
Ishigameyoh (gap) was identified as a spontaneous mutant of Bombyx mori with an apterous and sterile phenotype. Phenotypic observations revealed that the gap mutation affected the development of imaginal discs and the primordia of adult organs, including the wings, legs, compound eyes, and reproductive organs. Therefore, Ishigameyoh (gap) is a metamorphosis-defective mutant. We found that oogenesis depends on the gap gene, whereas spermatogenesis is independent. Ovary transplantation experiments suggest a cell-autonomous requirement for the gap gene in the process of oogenesis. The gap mutation has been maintained by crossing+ gap/gap moths as the u90 strain. However, the u90 strain is difficult to maintain because+ gap/+ gap and+ gap/gap moths cannot be distinguished phenotypically. For efficacious maintenance, we established a molecular marker that is closely linked to the gap locus. First, we localized the gap locus to chromosome 5 using phenotypic markers. Second, the gap locus was narrowed down to within a 1-Mb region using PCR-based markers. Within this 1-Mb region, we finally established a PCR-based marker that amplified different-sized products from+ gap/+ gap and gap/gap offspring of the u90 strain. This marker allows the distinction of+ gap/+ gap and+ gap/gap moths with an error rate of< 1% and therefore reduces the labor needed to maintain the u90 strain. Moreover, this marker helps to identify+ gap and gap individuals before pupation, which is necessary for larvae-based genetic and physiological analyses of the gap gene.
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家蚕白化突变体的遗传学研究
DOI：
10.11416/kontyushigen1930.42.411
发表时间：
1973
期刊：
The journal of sericultural science of Japan
影响因子：
--
作者：
H. Doira;Haruo Chikushi;H. Kihara
通讯作者：
H. Kihara
影响因子：
2.2
作者：
Allee, J. Paul;Pelletier, Chris L.;Champlin, David T.
通讯作者：
Champlin, David T.
影响因子：
56.9
作者：
Truman, JW;Hiruma, K;Riddiford, LM
通讯作者：
Riddiford, LM
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
Yutaka Banno;Yufuko Minohara;Maiko Kurakake;Junko Banno;Kazuhiro Nishikawa;Kei Tamura;Kazunori Yamamoto
通讯作者：
Kazunori Yamamoto