Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes.
Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes.
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作者：
Yang P;Chen Y;Huang Z;Xia H;Cheng L;Wu H;Zhang Y;Wang F
尽管先天免疫在无脊椎动物中很重要，但无脊椎动物中先天免疫细胞的多样性和功能在很大程度上还是未知的。使用单细胞 RNA-seq，我们确定了原血细胞、单核细胞和粒细胞是白虾血淋巴中的三种主要细胞类型。我们的结果发现了一个新的巨噬细胞样子集，称为单核血细胞 2 (MH2)，由某些标记基因（包括 Nlrp3 和 Casp1）的表达来定义。这种虾血细胞亚型具有吞噬能力，表达的标记表明与哺乳动物巨噬细胞存在一定的保守性。结合起来，我们的工作解决了一种在经济上非常重要的水生物种中血细胞的异质性，并确定了一种新的先天免疫细胞亚群，该亚群可能在虾对影响该行业的威胁传染病的免疫反应中发挥关键作用。
Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph. Our results identified a novel macrophage-like subset called monocytic hemocytes 2 (MH2) defined by the expression of certain marker genes, including Nlrp3 and Casp1. This subtype of shrimp hemocytes is phagocytic and expresses markers that indicate some conservation with mammalian macrophages. Combined, our work resolves the heterogenicity of hemocytes in a very economically important aquatic species and identifies a novel innate immune cell subset that is likely a critical player in the immune responses of shrimp to threatening infectious diseases affecting this industry.
影响因子：
7.3
作者：
Huang Z;Yang P;Wang F
通讯作者：
Wang F