Shrimp Plasma CREG Is a Hemocyte Activation Factor.
Shrimp Plasma CREG Is a Hemocyte Activation Factor.
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DOI：
10.3389/fimmu.2021.707770
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发表时间：
2021
影响因子：
7.3
通讯作者：
Wang F
中科院分区：
文献类型：
--
作者：
Huang Z;Yang P;Wang F
细胞因子是一类由细胞分泌的免疫调节蛋白。尽管脊椎动物细胞因子，特别是哺乳动物细胞因子的研究已经有几十年了。但对无脊椎动物的研究较少，因此在无脊椎动物中只发现了一些细胞因子。我们选择凡纳滨对虾作为探索新型无脊椎动物细胞因子的模型。为了实现这一点，我们以前纯化虾血浆低丰度蛋白，并确定了400多个蛋白质组学分析。在这项研究中，E1A刺激基因（CREG）样蛋白，这是高度保守的从黑腹果蝇到智人的细胞阻遏物，进一步在虾血浆中的特点。结果表明，对虾血浆CREG是一种糖蛋白，在注射LPS后8h，CREG在血淋巴中被强烈诱导。进一步的功能实验表明，重组对虾CREG蛋白注射液能显著提高血淋巴中吞噬性血细胞和溶酶体高血细胞的比例。然后，对注射rEGFP和rCREG蛋白的对虾血细胞进行转录组分析，结果表明，对虾CREG蛋白能全面促进血细胞的成熟和活化。综上所述，我们的数据清楚地表明，虾血浆CREG蛋白是一种新的血细胞激活因子，这可能是一个保守的骨髓细胞谱系激活因子从无脊椎动物到脊椎动物。
Cytokines are a class of immunoregulatory proteins that are secreted by cells. Although vertebrate cytokine, especially mammalian cytokine has been well studied for the past decades. Much less attention has been paid to invertebrate so that only some cytokines have been identified in invertebrates. We have chosen Peaneus vannamei as a model to explore novel invertebrate cytokines. To achieve this, we previously purified shrimp plasma low abundance proteins and identified more than 400 proteins with proteomics analyses. In this study, a cellular repressor of E1A-stimulated gene (CREG)-like protein, which is highly conserved from Drosophila melanogaster to Homo sapiens, was further characterized in shrimp plasma. We found that shrimp plasma CREG was a glycoprotein which was strongly induced in hemolymph at 8 h post-LPS injection. Further function experiment unveiled that recombinant shrimp CREG protein injection significantly increased phagocytic hemocyte and lysosome-high hemocyte proportion in hemolymph. After that, hemocytes from rEGFP- and rCREG-protein injected shrimps were subjected to transcriptome analyses, which revealed that shrimp CREG protein could comprehensively promote hemocyte maturation and activation. Taken together, our data clearly indicated that shrimp plasma CREG protein is a novel hemocyte activation factor, which is probably a conserved myeloid cell lineage activation factor from invertebrate to vertebrate.
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影响因子：
1.6
作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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影响因子：
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通讯作者：
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DOI：
10.1126/science.1190689
发表时间：
2010-09-10
期刊：
Science (New York, N.Y.)
影响因子：
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通讯作者：
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