Spatially Targeted Proteomics of the Host-Pathogen Interface during Staphylococcal Abscess Formation.
Spatially Targeted Proteomics of the Host-Pathogen Interface during Staphylococcal Abscess Formation.
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DOI：
10.1021/acsinfecdis.0c00647
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发表时间：
2021-01-08
影响因子：
5.3
通讯作者：
Spraggins JM
中科院分区：
文献类型：
--
作者：
Guiberson ER;Weiss A;Ryan DJ;Monteith AJ;Sharman K;Gutierrez DB;Perry WJ;Caprioli RM;Skaar EP;Spraggins JM
金黄色葡萄球菌是侵袭性和危及生命的感染的常见原因，通常具有多重耐药性。为了开发新的治疗方法，详细了解感染过程中复杂的宿主-病原体相互作用是必不可少的。对于控制组织脓肿形成的分子过程尤其如此，因为这些异质结构是葡萄球菌致病性的重要贡献者。为了充分表征导致成熟脓肿的发育过程，需要时间和空间分析方法。空间靶向蛋白质组学技术，如微液萃取表面分析，提供了对复杂生物系统的洞察，包括检测细菌蛋白质及其在宿主环境中的丰度。通过分析感染过程中不同脓肿区域的蛋白质组学成分，我们通过空间和时间蛋白质组学评估确定了免疫反应和细菌对脓肿发展的贡献。收集到的信息被映射到生化途径，以表征宿主的代谢过程和免疫策略。这些数据提供了对脓肿内细菌生理状态的见解，并阐明了宿主-病原体界面的致病过程。
Staphylococcus aureus is a common cause of invasive and life-threatening infections that are often multidrug resistant. To develop novel treatment approaches, a detailed understanding of the complex host–pathogen interactions during infection is essential. This is particularly true for the molecular processes that govern the formation of tissue abscesses, as these heterogeneous structures are important contributors to staphylococcal pathogenicity. To fully characterize the developmental process leading to mature abscesses, temporal and spatial analytical approaches are required. Spatially targeted proteomic technologies such as microliquid extraction surface analysis offer insight into complex biological systems including detection of bacterial proteins and their abundance in the host environment. By analyzing the proteomic constituents of different abscess regions across the course of infection, we defined the immune response and bacterial contribution to abscess development through spatial and temporal proteomic assessment. The information gathered was mapped to biochemical pathways to characterize the metabolic processes and immune strategies employed by the host. These data provide insights into the physiological state of bacteria within abscesses and elucidate pathogenic processes at the host–pathogen interface.
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