A single-cell atlas of murine reproductive tissues during preterm labor.
A single-cell atlas of murine reproductive tissues during preterm labor.
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DOI：
10.1016/j.celrep.2022.111846
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发表时间：
2023-01-31
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
早产是世界范围内围产儿发病率和死亡率的主要原因，常由早产综合征引起。最确定的早产的因果关系是羊膜内感染，它涉及生殖组织中分娩级联的过早激活。在此，我们利用单细胞RNA测序（scRNA-seq）在感染诱导的早产模型中生成小鼠子宫、蜕膜和宫颈的单细胞图谱。我们表明，早产影响特定的免疫和非免疫细胞亚群的转录谱。在受影响的细胞中鉴定出共享的和组织特异性的基因表达特征。细胞间通讯的测定暗示了跨组织的早产相关信号通路中的特定细胞类型。小鼠和人类子宫细胞间相互作用的计算机比较揭示了与分娩有关的保守信号通路。因此，我们的scRNA-seq数据为早产驱动的细胞景观和生殖组织中的通信提供了见解。Garcia-Flores等人使用羊膜内感染诱导的早产模型生成了小鼠子宫、蜕膜和宫颈的单细胞图谱，并证明了细胞类型组成、转录谱和细胞-细胞信号传导的改变。这个scRNA-seq数据集可以作为未来研究的宝贵资源。
Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the syndrome of preterm labor. The best-established causal link to preterm labor is intra-amniotic infection, which involves premature activation of the parturition cascade in the reproductive tissues. Herein, we utilize single-cell RNA sequencing (scRNA-seq) to generate a single-cell atlas of the murine uterus, decidua, and cervix in a model of infection-induced preterm labor. We show that preterm labor affects the transcriptomic profiles of specific immune and non-immune cell subsets. Shared and tissue-specific gene expression signatures are identified among affected cells. Determination of intercellular communications implicates specific cell types in preterm labor-associated signaling pathways across tissues. In silico comparison of murine and human uterine cell-cell interactions reveals conserved signaling pathways implicated in labor. Thus, our scRNA-seq data provide insights into the preterm labor-driven cellular landscape and communications in reproductive tissues. Garcia-Flores et al. generate a single-cell atlas of the murine uterus, decidua, and cervix using a model of intra-amniotic infection-induced preterm labor and demonstrate alterations in cell type composition, transcriptional profiles, and cell-cell signaling. This scRNA-seq dataset can serve as a valuable resource to be leveraged by future investigations.
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