The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis.
The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis.
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DOI：
10.3390/ijms222212434
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发表时间：
2021-11-18
影响因子：
5.6
通讯作者：
Yoshimura A
中科院分区：
文献类型：
--
作者：
Mae M;Alam MI;Yamashita Y;Ozaki Y;Higuchi K;Ziauddin SM;Montenegro Raudales JL;Sakai E;Tsukuba T;Yoshimura A
牙结石是牙周炎患者常见的存款。我们先前已经表明，DC含有微生物成分和磷酸钙晶体，其通过巨噬细胞中的NLRP 3炎性体诱导破骨细胞生成细胞因子IL-1β。在这项研究中，我们研究了由小鼠巨噬细胞产生的细胞因子与DC刺激破骨细胞生成的影响。用DC刺激的野生型（WT）小鼠巨噬细胞的培养上清液加速RANKL引发的小鼠骨髓巨噬细胞（BMSCs）中的破骨细胞生成，但抑制RANKL引发的RAW-D细胞中的破骨细胞生成。用DC刺激的WT而非NLRP 3缺陷型小鼠巨噬细胞以剂量依赖性方式产生IL-1β和IL-18，表明IL-1β和IL-18的NLRP 3炎性小体依赖性产生。用DC刺激的WT和NLRP 3缺陷型小鼠巨噬细胞均产生IL-10，表明IL-10的NLRP 3炎性小体非依赖性产生。重组IL-1β可加速RANKL致敏的Bronze和RAW-D细胞中的破骨细胞生成，而重组IL-18和IL-10可抑制破骨细胞生成。这些结果表明，DC以NLRP 3炎性体依赖性方式诱导破骨细胞生成IL-1β，并且分别依赖于和独立于NLRP 3炎性体诱导抗成骨IL-18和IL-10。DC可能通过IL-1β诱导促进牙周炎患者牙槽骨吸收，但在某些情况下可能通过IL-18和IL-10诱导抑制牙槽骨吸收。
Dental calculus (DC) is a common deposit in periodontitis patients. We have previously shown that DC contains both microbial components and calcium phosphate crystals that induce an osteoclastogenic cytokine IL-1β via the NLRP3 inflammasome in macrophages. In this study, we examined the effects of cytokines produced by mouse macrophages stimulated with DC on osteoclastogenesis. The culture supernatants from wild-type (WT) mouse macrophages stimulated with DC accelerated osteoclastogenesis in RANKL-primed mouse bone marrow macrophages (BMMs), but inhibited osteoclastogenesis in RANKL-primed RAW-D cells. WT, but not NLRP3-deficient, mouse macrophages stimulated with DC produced IL-1β and IL-18 in a dose-dependent manner, indicating the NLRP3 inflammasome-dependent production of IL-1β and IL-18. Both WT and NLRP3-deficient mouse macrophages stimulated with DC produced IL-10, indicating the NLRP3 inflammasome-independent production of IL-10. Recombinant IL-1β accelerated osteoclastogenesis in both RANKL-primed BMMs and RAW-D cells, whereas recombinant IL-18 and IL-10 inhibited osteoclastogenesis. These results indicate that DC induces osteoclastogenic IL-1β in an NLRP3 inflammasome-dependent manner and anti-osteogenic IL-18 and IL-10 dependently and independently of the NLRP3 inflammasome, respectively. DC may promote alveolar bone resorption via IL-1β induction in periodontitis patients, but suppress resorption via IL-18 and IL-10 induction in some circumstances.
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DOI：
10.1073/pnas.0812690106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Cayrol, Corinne;Girard, Jean-Philippe
通讯作者：
Girard, Jean-Philippe
影响因子：
3.7
作者：
Montenegro Raudales JL;Yoshimura A;Sm Z;Kaneko T;Ozaki Y;Ukai T;Miyazaki T;Latz E;Hara Y
通讯作者：
Hara Y
影响因子：
8.7
作者：
Dinarello CA
通讯作者：
Dinarello CA
影响因子：
3.9
作者：
Fons-Badal, Carla;Fons-Font, Antonio;Agustin-Panadero, Ruben
通讯作者：
Agustin-Panadero, Ruben
影响因子：
4.4
作者：
Kitaura, Hideki;Tatamiya, Mutsuhito;Nakayama, Koji
通讯作者：
Nakayama, Koji