Cell-based biosensor to report DNA damage in micro- and nanosystems.
Cell-based biosensor to report DNA damage in micro- and nanosystems.
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DOI：
10.1021/ac501412c
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发表时间：
2014-08-05
影响因子：
7.4
通讯作者：
Voldman, Joel
中科院分区：
文献类型：
--
作者：
Fendyur, Anna;Varma, Sarvesh;Lo, Catherine T.;Voldman, Joel
了解新设计的与细胞相互作用的微米和纳米级材料和系统如何影响细胞生理学，对于开发和最终采用此类技术至关重要。关于在这种可直接或间接损伤DNA的系统中施加于细胞的力的遗传毒性影响的报告强调，需要开发简便的方法来评估材料和技术如何影响细胞生理学。为了满足这一需求，我们在NIH-3T3细胞中开发了一种基于TurboRFP的DNA损伤报告细胞系，该细胞系可以荧光报告从化学遗传毒剂到UV-C辐射等各种因素造成的遗传毒性应激。我们的生物传感器被成功地应用于报告纳米材料的遗传毒性影响，展示了评估大小依赖的基因和细胞毒性的能力。生物传感器细胞可以以高通量、非侵入性的方式进行检测，不需要过于复杂的设备或额外的试剂。我们相信，这种开源的生物传感器是微纳材料社区以及希望评估系统和材料对细胞生理学影响的系统设计师和用户的重要资源。
Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize the need for developing facile methods to assess how materials and technologies affect cell physiology. To address this need we have developed a TurboRFP-based DNA damage reporter cell line in NIH-3T3 cells that fluoresce to report genotoxic stress caused by a wide variety of agents, from chemical genotoxic agents to UV-C radiation. Our biosensor was successfully implemented in reporting the genotoxic impact of nanomaterials, demonstrating the ability to assess size dependent geno- and cyto-toxicity. The biosensor cells can be assayed in a high throughput, noninvasive manner, with no need for overly sophisticated equipment or additional reagents. We believe that this open-source biosensor is an important resource for the community of micro- and nanomaterials and systems designers and users who wish to evaluate the impact of systems and materials on cell physiology.
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DOI：
10.1016/j.mrgentox.2006.04.011
发表时间：
2006-09-05
影响因子：
1.9
作者：
Hastwell, Paul W.;Chai, Li-Leng;Walmsley, Richard M.
通讯作者：
Walmsley, Richard M.
影响因子：
3.2
作者：
Hattinger, CM;Jochemsen, AG;Dirks, RW
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影响因子：
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作者：
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DOI：
10.3389/fneng.2011.00014
发表时间：
2011
期刊：
Frontiers in neuroengineering
影响因子：
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作者：
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通讯作者：
Spira ME
影响因子：
38.3
作者：
Bhabra, Gevdeep;Sood, Aman;Case, Charles Patrick
通讯作者：
Case, Charles Patrick