LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate.
LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate.
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DOI：
10.1038/s41467-020-20883-w
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发表时间：
2021-01-27
影响因子：
16.6
通讯作者：
Wagner D
中科院分区：
文献类型：
--
作者：
Jin R;Klasfeld S;Zhu Y;Fernandez Garcia M;Xiao J;Han SK;Konkol A;Wagner D
多细胞真核生物中的主转录因子重编程细胞命运。先锋转录因子在这一过程中具有突出的作用，因为它们能够在封闭的染色质中接触它们的同源结合基序。重编程在植物中普遍存在，其发育是可塑的，并受环境调节，但对这个王国中的先驱转录因子知之甚少。在这里，我们表明，主转录因子叶（LFY），促进花的命运，通过上调花的承诺因子APETALA 1（AP 1），是一个先驱转录因子。在体外，LFY与组装成核小体的内源性AP 1靶基因座DNA结合。在体内，LFY与核小体在其大多数靶位点（包括AP 1）上占据的结合位点相关联。在结合后，LFY '解锁'染色质局部取代H1接头组蛋白，并通过招募SWI/SNF染色质重塑，但广泛的变化，染色质可及性发生后。我们的研究提供了一个花序结构模式化的机制框架，并揭示了LFY和动物先锋转录因子之间惊人的相似之处。先锋转录因子在封闭的染色质中访问它们的DNA结合基序，并且通常在细胞命运重编程中起作用。在这里，Jin等人提出了植物中先锋因子的生化证据，并表明LFY促进花细胞命运，并通过取代组蛋白H1和招募SWI/SNF染色质重塑剂来局部解锁染色质。
Master transcription factors reprogram cell fate in multicellular eukaryotes. Pioneer transcription factors have prominent roles in this process because of their ability to contact their cognate binding motifs in closed chromatin. Reprogramming is pervasive in plants, whose development is plastic and tuned by the environment, yet little is known about pioneer transcription factors in this kingdom. Here, we show that the master transcription factor LEAFY (LFY), which promotes floral fate through upregulation of the floral commitment factor APETALA1 (AP1), is a pioneer transcription factor. In vitro, LFY binds to the endogenous AP1 target locus DNA assembled into a nucleosome. In vivo, LFY associates with nucleosome occupied binding sites at the majority of its target loci, including AP1. Upon binding, LFY ‘unlocks’ chromatin locally by displacing the H1 linker histone and by recruiting SWI/SNF chromatin remodelers, but broad changes in chromatin accessibility occur later. Our study provides a mechanistic framework for patterning of inflorescence architecture and uncovers striking similarities between LFY and animal pioneer transcription factor. Pioneer transcription factors access their DNA binding motifs in closed chromatin and often act in cell fate reprogramming. Here, Jin et al. present biochemical evidence for a pioneer factor in plants and show that LFY promotes floral cell fate and locally unlocks chromatin by displacing histone H1 and recruiting SWI/SNF chromatin remodelers.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
7.2
作者：
Benlloch, Reyes;Kim, Min Chul;Nilsson, Ove
通讯作者：
Nilsson, Ove
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
30.8
作者：
Donaghey J;Thakurela S;Charlton J;Chen JS;Smith ZD;Gu H;Pop R;Clement K;Stamenova EK;Karnik R;Kelley DR;Gifford CA;Cacchiarelli D;Rinn JL;Gnirke A;Ziller MJ;Meissner A
通讯作者：
Meissner A
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF