Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice
Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice
复制标题
转基因小鼠猿病毒40大肿瘤抗原表达与肿瘤形成的关系
作者：
T. Dyke;C. Finlay;D. Miller;J. Marks;G. Lozano;A. Levine
在病毒增强子-启动子的控制下，一组含有猴病毒40(SV40)大肿瘤抗原基因的转基因小鼠在出生后的头两周内在这些小鼠的大脑中表达了这种病毒蛋白。出生后36~41d，这些小鼠的脉络膜神经丛中形成多个未分化细胞灶，正常组织与这些转化灶共存。免疫过氧化物酶染色检测SV40T抗原显示，在肿瘤发展的晚期，大约90-100天及之后，核T抗原表达具有肿瘤特异性。在肿瘤发展的早期，绝大多数脉络丛细胞的SV40T抗原水平在每个细胞的基础上似乎较低。这些结果表明，在可发现的病理之前(36至41天)，肿瘤抗原水平较低(14~36天)，而在快速生长的晚期肿瘤(大于90天)中，每个细胞的T抗原水平较高。
A line of transgenic mice containing the simian virus 40 (SV40) large tumor antigen gene under the control of the viral enhancer-promoter expressed this viral protein in the brains of these mice within the first 2 weeks after birth. Multiple foci of anaplastic cells formed in the choroid plexuses of these mice at 36 to 41 days after birth, and normal tissue coexisted with these transformed foci. Immunoperoxidase staining to detect the SV40 T antigen showed tumor-specific expression of nuclear T antigen at late times in tumor development, approximately 90 to 100 days and thereafter. The level of SV40 T antigen, on a per cell basis, appeared to be lower in the great majority of choroid plexus cells at earlier times in tumor development. These results suggest that low levels of tumor antigen (14 to 36 days) are present before detectable pathology (36 to 41 days) and the level of T antigen per cell is higher in rapidly growing late-stage tumors (older than 90 days).
几个含有 SV40 早期基因的转基因小鼠品系的比较。
DOI：
10.1101/sqb.1985.050.01.082
发表时间：
1985
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
VanDyke,T;Finlay,C;Levine,AJ
通讯作者：
Levine,AJ
弹性蛋白酶 I 启动子指导转基因小鼠的胰腺腺泡细胞表达人类生长激素和 SV40 T 抗原基因。
DOI：
10.1101/sqb.1985.050.01.050
发表时间：
1985
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Ornitz,DM;Palmiter,RD;Messing,A;Hammer,RE;Pinkert,CA;Brinster,RL
通讯作者：
Brinster,RL