A novel α/β-hydrolase gene IbMas enhances salt tolerance in transgenic sweetpotato.
A novel α/β-hydrolase gene IbMas enhances salt tolerance in transgenic sweetpotato.
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新型 a/b-水解酶基因 IbMas 增强转基因甘薯的耐盐性
DOI：
10.1371/journal.pone.0115128
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Liu D;Wang L;Zhai H;Song X;He S;Liu Q
盐胁迫是世界范围内农业生产中的主要环境胁迫之一，影响作物的产量和品质。因此，发展耐盐性水平较高的作物是非常可取的。本研究从耐盐性甘薯(Ipomoea Batatas(L.)LAM.)ND98线。IBMA含有Masparin结构域，属于α/β-水解酶超家族。盐胁迫和ABA处理上调了IBMAs在甘薯中的表达。高表达甘薯(cv.与野生型相比，上薯植株表现出更高的耐盐性。转基因植株中的脯氨酸含量显著增加，而丙二醛含量显著降低。转基因植株的超氧化物歧化酶(SOD)活性和光合作用显著增强。转基因植株中过氧化氢的积累量也明显低于野生型植株。在盐胁迫下，IBMA的过表达上调了盐胁迫响应基因，包括吡咯烷-5-羧酸合成酶、吡咯烷-5-羧酸还原酶、超氧化物歧化酶、PSBA和磷酸核糖核酸激酶基因。这些结果表明，IBMAs的过度表达通过调节渗透平衡、保护膜完整性和光合作用以及提高对活性氧的清除能力来提高转基因甘薯的耐盐性。
Salt stress is one of the major environmental stresses in agriculture worldwide and affects crop productivity and quality. The development of crops with elevated levels of salt tolerance is therefore highly desirable. In the present study, a novel maspardin gene, named IbMas, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbMas contains maspardin domain and belongs to α/β-hydrolase superfamily. Expression of IbMas was up-regulated in sweetpotato under salt stress and ABA treatment. The IbMas-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbMas up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes, under salt stress. These findings suggest that overexpression of IbMas enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.
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影响因子：
2.6
作者：
Liu, QC;Zhai, H;Zhang, DP
通讯作者：
Zhang, DP
甘薯碳水化合物代谢相关基因 IbSnRK1 的克隆和表征
影响因子：
4.3
作者：
Jiang, Tao;Zhai, Hong;Liu, Qingchang
通讯作者：
Liu, Qingchang
影响因子：
4.3
作者：
De Ronde, JA;Cress, WA;Van Staden, J
通讯作者：
Van Staden, J
影响因子：
6.2
作者：
Dhir, SK;Oglesby, J;Bhagsari, AS
通讯作者：
Bhagsari, AS
影响因子：
4.3
作者：
Fernández-Falcón, M;Hernández, M;Borges, AA
通讯作者：
Borges, AA