miR-29b and miR-125a regulate podoplanin and suppress invasion in glioblastoma.
miR-29b and miR-125a regulate podoplanin and suppress invasion in glioblastoma.
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DOI：
10.1002/gcc.20808
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Calin GA
中科院分区：
文献类型：
--
作者：
Cortez MA;Nicoloso MS;Shimizu M;Rossi S;Gopisetty G;Molina JR;Carlotti C Jr;Tirapelli D;Neder L;Brassesco MS;Scrideli CA;Tone LG;Georgescu MM;Zhang W;Puduvalli V;Calin GA
胶质母细胞瘤是最常见的恶性脑肿瘤，其特征在于细胞增殖和侵袭能力的提高。近年来研究表明，PDPN基因编码的podoplanin膜唾液酸糖蛋白在星形细胞肿瘤中过表达，并与肿瘤侵袭有关，但其调控机制尚不清楚。MicroRNA是一种非编码RNA，可调节基因表达和多种生物学过程和疾病，包括癌症。然而，它们在胶质母细胞瘤的侵袭、增殖和凋亡中的作用尚不完全清楚。在本研究中，我们重点研究了miR-29 b和miR-125 a，它们被预测为调节PDPN，并证明这些microRNA直接靶向PDPN的3′非翻译区，并抑制胶质母细胞瘤的侵袭、凋亡和增殖。此外，我们报告了miR-29 b和miR-125 a在胶质母细胞瘤和CD 133阳性细胞中下调。总之，这些结果表明miR-29 b和miR-125 a代表胶质母细胞瘤的潜在治疗靶点。
Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation and invasion. Recently, it was demonstrated that podoplanin membrane sialo-glycoprotein encoded by PDPN gene is over-expressed and related to cellular invasion in astrocytic tumors; however the mechanisms of regulation are still unknown. MicroRNAs are noncoding RNAs that regulate gene expression and several biological processes and diseases, including cancer. Nevertheless, their roles in invasion, proliferation, and apoptosis of glioblastoma are not completely understood. In this study, we focused on miR-29b and miR-125a, which were predicted to regulate PDPN, and demonstrated that these microRNAs directly target the 3′ untranslated region of PDPN and inhibit invasion, apoptosis, and proliferation of glioblastomas. Furthermore, we report that miR-29b and miR-125a are downregulated in glioblastomas and also in CD133-positive cells. Taken together, these results suggest that miR-29b and miR-125a represent potential therapeutic targets in glioblastoma.
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影响因子：
5.2
作者：
Krichevsky, Anna M.;Sonntag, Kai-C.;Kosik, Kenneth S.
通讯作者：
Kosik, Kenneth S.
DOI：
10.1073/pnas.0700071104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Laneve, Pietro;Di Marcotullio, Lucia;Caffarelli, Elisa
通讯作者：
Caffarelli, Elisa
影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM
影响因子：
11.2
作者：
Godlewski, Jakub;Nowicki, Michal O.;Lawler, Sean
通讯作者：
Lawler, Sean
影响因子：
8
作者：
Mott, J. L.;Kobayashi, S.;Gores, G. J.
通讯作者：
Gores, G. J.