Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish.
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DOI：
10.1371/journal.pone.0031658
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin S
中科院分区：
文献类型：
--
作者：
Gomez G;Lee JH;Veldman MB;Lu J;Xiao X;Lin S
转录因子etsrp/Er71/ETV2是迄今为止研究的所有物种中血管生成的主控基因。它也是斑马鱼和小鼠造血所必需的。通过基因芯片对过度表达etsrp的斑马鱼胚胎进行转录图谱分析，发现了几个在维管系统中表达的新基因。在这里，我们用Illumina RNA测序技术重新检查了这一转录图谱，揭示了受etsrp调控的候选基因的数量大幅增加。对这个数据集中的50个候选基因进行的表达研究发现了39个在血管细胞中表达的新基因。ETSRP异位诱导ETSRP过表达和ETSRP缺陷胚胎中表达降低证实了ETSRP对这些基因的调控。我们的研究证明了RNA测序技术在斑马鱼中识别生物相关基因的有效性，并产生了以前在血管内皮细胞生物学中未曾探索过的基因的全面图谱。
The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It is also required for hematopoiesis in zebrafish and mice. Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. Regulation of these genes by etsrp was confirmed by their ectopic induction in etsrp overexpressing and decreased expression in etsrp deficient embryos. Our studies demonstrate the effectiveness of the RNA-sequencing technology to identify biologically relevant genes in zebrfish and produced a comprehensive profile of genes previously unexplored in vascular endothelial cell biology.
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