Difference in serum complement component C4a levels between hepatitis C virus carriers with persistently normal alanine aminotransferase levels or chronic hepatitis C.
Difference in serum complement component C4a levels between hepatitis C virus carriers with persistently normal alanine aminotransferase levels or chronic hepatitis C.
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DOI：
10.3892/mmr.2012.924
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发表时间：
2012-08
影响因子：
3.4
通讯作者：
Tsubouchi H
中科院分区：
文献类型：
--
作者：
Imakiire K;Uto H;Sato Y;Sasaki F;Mawatari S;Ido A;Shimoda K;Hayashi K;Stuver SO;Ito Y;Okanoue T;Tsubouchi H
某些丙型肝炎病毒携带者的丙氨酸氨基转移酶(ALT)水平持续正常(≤30 IU/L)，同时血小板计数正常(≥15×10~4/μL)；这些人表现出较轻的疾病活动性和较慢的肝硬变进展。本研究旨在用血清蛋白质组学方法阐明伴有PNALT的丙型肝炎病毒携带者的特征。第一组在医院接受临床评估，包括19名PNALT(PNALT-1)丙型肝炎病毒携带者(PNALT-1)和20名慢性丙型肝炎(CHC-1)患者。第二组受试者是肝病自然病史队列研究的一部分，包括37名PNALT(PNALT-2)和30名CHC(CHC-2)患者。亲和微珠纯化的血清蛋白进行基质辅助激光解吸电离飞行时间质谱仪分析。血清蛋白质组学显示，PNALT-1组和CHC-1组的6个蛋白质峰的质量/电荷比从1,000到3,000之间存在显著差异。在这些峰中，一个1738m/z的峰蛋白被鉴定为补体成分4(C4)的片段，并与酶免疫法测定的血清C4a浓度显著相关。PNALT-2组的血清C4a水平也显著高于CHC-2组和健康志愿者。此外，在PNALT-2组中，血清C4a水平与转氨酶水平呈负相关，但与其他生化指标、丙型肝炎病毒核心抗原水平、外周血细胞计数或血清肝纤维化标志物无关。这项研究表明，宿主因素如C4a不仅在PNALT和CHC携带者之间存在差异，而且随着更多差异的发现，蛋白质组学方法也可能有助于阐明PNALT中的因素。
Certain hepatitis C virus (HCV) carriers exhibit persistently normal alanine aminotransferase (ALT) levels (PNALT) (≤30 IU/l) accompanied by normal platelet counts (≥15×104/μl); these individuals show milder disease activity and slower progression to cirrhosis. This study aimed to elucidate the characteristics of HCV carriers with PNALT using serum proteomics. The first group of subjects, who underwent clinical evaluation in the hospital, consisted of 19 HCV carriers with PNALT (PNALT-1) and 20 chronic hepatitis C (CHC-1) patients. The second group of subjects was part of a cohort study on the natural history of liver disease, and included 37 PNALT (PNALT-2) and 30 CHC (CHC-2) patients. Affinity bead-purified serum protein was subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis. Serum proteomics showed that 6 protein peaks with mass-to-charge ratios ranging from 1,000 to 3,000 differed significantly between the PNALT-1 and CHC-1 groups. Among these peaks, a 1738-m/z peak protein was identified as a fragment of complement component 4 (C4) and correlated significantly with serum C4a concentrations as determined by enzyme immunoassay. Serum C4a levels were also significantly higher in the PNALT-2 group compared to the CHC-2 group and healthy volunteers. Furthermore, in the PNALT-2 group, serum C4a levels negatively correlated with transaminase levels, but not with other biochemical tests, HCV core antigen levels, peripheral blood cell counts or serum hepatic fibrosis markers. This study indicates that host factors such as C4a not only differ between HCV carriers with PNALT and CHC, but that proteomic approaches could also contribute to the elucidation of factors in PNALT as more differences are discovered.
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影响因子：
13.5
作者：
Uto, Hirofumi;Stuver, Sherri O.;Tsubouchi, Hirohito
通讯作者：
Tsubouchi, Hirohito
影响因子：
13.5
作者：
Kanmura, Shuji;Uto, Hirofumi;Tsubouchi, Hirohito
通讯作者：
Tsubouchi, Hirohito
影响因子：
2
作者：
Uto H;Kanmura S;Takami Y;Tsubouchi H
通讯作者：
Tsubouchi H
影响因子：
4.4
作者：
Ambrus, G;Gál, P;Závodszky, P
通讯作者：
Závodszky, P
影响因子：
12.7
作者：
Arase, Yasuji;Ikeda, Kenji;Kumada, Hiromitsu
通讯作者：
Kumada, Hiromitsu