Bioassays of compounds with potential juvenoid activity on Drosophila melanogaster: juvenile hormone III, bisepoxide juvenile hormone III and methyl farnesoates.
Bioassays of compounds with potential juvenoid activity on Drosophila melanogaster: juvenile hormone III, bisepoxide juvenile hormone III and methyl farnesoates.
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DOI：
10.1016/j.jinsphys.2010.06.003
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发表时间：
2010-10
影响因子：
2.2
通讯作者：
Hammock, Bruce D.
中科院分区：
文献类型：
--
作者：
Harshman, Lawrence G.;Song, Ki-Duck;Casas, Josephina;Schuurmans, A.;Kuwano, Eichii;Kachman, Stephen D.;Riddiford, Lynn M.;Hammock, Bruce D.
用果蝇（Drosophilamelanogaster）的早期幼虫或早期蛹测定了6，7，10，11双环氧化物保幼激素Ⅲ（JHB_3）的代谢产物和其它潜在的保幼激素活性。重要的是，测试了金合欢酸甲酯，因为它们可能对双翅目幼虫具有JH样活性。将幼虫暴露于培养基中的化合物，或将化合物施用于白色蛹。在本研究中使用的测定中，没有迹象表明JHB 3的代谢产物具有JH活性。法呢酸甲酯（MF）的活性高于JH Ⅲ，远大于双环氧化物JH Ⅲ。与内源性保幼激素不同，金合欢酸甲酯在白色蛹期生物测定中活性较弱。当氟形式的甲基法呢酸，这是不太可能被转化为JH，被施加到果蝇培养基中的苍蝇卵被引入，有一个高度的幼虫死亡率，但没有证据表明，随后的死亡率在蛹阶段。对该结果的一种可能的解释是，法尼酸甲酯在幼虫阶段作为激素是有活性的，但在蛹阶段几乎没有活性，其中只有保幼激素具有主要作用。
Metabolites of the 6,7,10,11 bisepoxide juvenile hormone III (JHB3), and other potential juvenoids, were tested for juvenile hormone activity using early instar or early stage pupae of Drosophila melanogaster. Importantly, methyl farnesoates were tested as they might have JH-like activity on Dipteran juveniles. Larvae were exposed to compounds in medium, or the compounds were applied to white puparia. In the assays employed in the present study, there was no indication for JH activity associated with the metabolites of JHB3. The activity of methyl farnesoate (MF) was higher than that of JH III and far greater than bisepoxide JH III. As opposed to the two endogenous juvenile hormones, methyl farnesoate has weak activity in the white puparial bioassaay. When fluorinated forms of methyl farnesoate, which is unlikely to be converted to JH, were applied to Drosophila medium to which fly eggs were introduced, there was a high degree of larval mortality, but no evidence of subsequent mortality at the pupal stage. One possible explanation for the results is that methyl farnesoate is active as a hormone in larval stages, but has little activity at the pupal stage where only juvenile hormone has a major effect.
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影响因子：
2.7
作者：
SAUNDERS, DS;RICHARD, DS;GILBERT, LI
通讯作者：
GILBERT, LI
影响因子：
2.7
作者：
RIDDIFORD, LM;ASHBURNER, M
通讯作者：
ASHBURNER, M
影响因子：
5.4
作者：
Jones, Grace;Jones, Davy;Wozniak, Mietek
通讯作者：
Wozniak, Mietek
影响因子：
2.1
作者：
MESSEGUER, A;SANCHEZBAEZA, F;HAMMOCK, BD
通讯作者：
HAMMOCK, BD
影响因子：
1.6
作者：
POSTLETHWAIT, JH
通讯作者：
POSTLETHWAIT, JH