Genetic dissection of rice grain shape using a recombinant inbred line population derived from two contrasting parents and fine mapping a pleiotropic quantitative trait locus qGL7.
Genetic dissection of rice grain shape using a recombinant inbred line population derived from two contrasting parents and fine mapping a pleiotropic quantitative trait locus qGL7.
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使用源自两个对比亲本的重组自交系群体对稻粒形状进行遗传解剖，并精细定位多效性数量性状基因座 qGL7
DOI：
10.1186/1471-2156-11-16
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发表时间：
2010-02-26
期刊：
影响因子：
2.9
通讯作者：
Xing Y
中科院分区：
文献类型：
--
作者：
Bai X;Luo L;Yan W;Kovi MR;Zhan W;Xing Y
背景稻米的三维形状是稻米外观的重要组成部分，其测量指标为稻米的长度、宽度和厚度(GL、GW和GT)。确定籽粒形状变异的遗传基础有助于有效地改善籽粒外观。本研究利用水稻籽粒大小不同的杂交组合南阳占和川7号的F7：8重组自交系群体(RIL)进行数量性状座位(QTL)定位。构建了164个SSR标记的遗传连锁图谱。结果在第3、7染色体上检测到4个控制粒形的QTL，在第2、3、5、7、9、10染色体上分别检测到10个GW和9个GT QTL。共检测到28个QTL，其中有几个为首次报道；两年中普遍检测到4个主效QTL和6个微效QTL。QTL qGL7对GL、GW、GT、千粒重(TGW)和每穗粒数(SPP)具有多效性效应，并在近等基因F2群体(NIL-F2)中得到进一步验证。最后将qGL7缩小到Indel标记RID711和SSR标记RM6389之间，覆盖了日本晴基因组中258 kb的区域，并与Indel标记RID710和RID76共分离。结论表型差异很大的材料由于遗传背景复杂，可用于QTL的定位群体。子代检测证明，该QTL qGL7表现出单一的孟德尔特征。因此，我们建议在一个较大的近等基因系F2群体中，利用基于图谱的克隆策略，分离到遗传力较高的性状的微效QTL。此外，不同QTL的组合产生了不同的粒形，这为培育更多的水稻品种以满足消费者的偏好提供了能力。
BackgroundThe three-dimensional shape of grain, measured as grain length, width, and thickness (GL, GW, and GT), is one of the most important components of grain appearance in rice. Determining the genetic basis of variations in grain shape could facilitate efficient improvements in grain appearance. In this study, an F7:8recombinant inbred line population (RIL) derived from a cross betweenindicaandjaponicacultivars (Nanyangzhan and Chuan7) contrasting in grain size was used for quantitative trait locus (QTL) mapping. A genetic linkage map was constructed with 164 simple sequence repeat (SSR) markers. The major aim of this study was to detect a QTL for grain shape and to fine map a minor QTL,qGL7.ResultsFour QTLs for GL were detected on chromosomes 3 and 7, and 10 QTLs for GW and 9 QTLs for GT were identified on chromosomes 2, 3, 5, 7, 9 and 10, respectively. A total of 28 QTLs were identified, of which several are reported for the first time; four major QTLs and six minor QTLs for grain shape were also commonly detected in both years. The minor QTL,qGL7, exhibited pleiotropic effects on GL, GW, GT, 1000-grain weight (TGW), and spikelets per panicle (SPP) and was further validated in a near isogenic F2population (NIL-F2). Finally,qGL7was narrowed down to an interval between InDel marker RID711 and SSR marker RM6389, covering a 258-kb region in the Nipponbare genome, and cosegregated with InDel markers RID710 and RID76.ConclusionMaterials with very different phenotypes were used to develop mapping populations to detect QTLs because of their complex genetic background. Progeny tests proved that the minor QTL,qGL7, could display a single mendelian characteristic. Therefore, we suggested that minor QTLs for traits with high heritability could be isolated using a map-based cloning strategy in a large NIL-F2population. In addition, combinations of different QTLs produced diverse grain shapes, which provide the ability to breed more varieties of rice to satisfy consumer preferences.
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