RNA-Seq-based transcriptome analysis of stem development and dwarfing regulation in Agapanthus praecox ssp. orientalis (Leighton) Leighton.
RNA-Seq-based transcriptome analysis of stem development and dwarfing regulation in Agapanthus praecox ssp. orientalis (Leighton) Leighton.
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基于 RNA-Seq 的百子莲茎发育和矮化调控转录组分析。
DOI：
10.1016/j.gene.2015.04.013
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发表时间：
2015-07
期刊：
影响因子：
3.5
通讯作者：
Shen, Xiao-Hui
中科院分区：
文献类型：
--
作者：
Shi, Yu-Bo;Zhuo, Li-Huan;Wang, Ling;Shen, Xiao-Hui
百子莲是单子叶观赏鳞茎植物。一般情况下，花茎长度可发育到1 m以上，但施用400 mg·L-1多效唑可使花茎长度缩短70%以上。为了更深入地了解其致矮机制，我们首次采用从头RNA测序技术对A.早熟共获得71，258个组装的unigenes，其中45，597个unigenes获得了蛋白质功能注释。以上述测序结果作为参考基因集，利用RNA-seq（quantitative）技术分析对照和多效唑处理样品间的基因表达谱，筛选出2838个差异表达基因。GO、KEGG和MapMan通路分析表明，这些差异表达基因在刺激、激素信号、糖代谢、细胞壁、细胞大小和细胞周期相关的生物学过程中显著富集。为了验证通过RNA-Seq获得的表达谱，对选自关键显著富集途径的24个基因进行实时qPCR。综合分析认为，多效唑阻断了能有效抑制花葶伸长的GA信号，GA信号与生长素、乙烯、油菜素内酯和细胞分裂素等激素信号相互作用，触发下游信号级联反应，导致代谢、细胞壁合成、细胞分裂和细胞周期明显下降，最终诱导矮化性状。此外，AP 2/EREBP、bHLH、C2 H2、ARR、WRKY和ARF家族的转录因子参与了花葶发育的调控。早熟该转录组数据集将作为一个重要的公共信息平台，加速百子莲基因表达和功能基因组学的研究。
Agapanthuspraecoxis a monocotyledonous ornamental bulb plant. Generally, the scape (inflorescence stem) length can develop more than 1 m, however application 400 mg·L− 1paclobutrazol can shorten the length beyond 70%. To get a deeper insight into its dwarfism mechanism, de novo RNA-Seq technology has been employed, for the first time, to describe the scape transcriptome ofA. praecox. We got 71,258 assembled unigenes, and 45,597 unigenes obtained protein functional annotation. Take the above sequencing results as a reference gene set, using RNA-seq (quantification) technology analyzed gene expression profiles between the control and paclobutrazol-treated samples, and screened 2838 differentially expressed genes. GO, KEGG and MapMan pathway analyses indicated that these differentially expressed genes were significantly enriched in response to stimulus, hormonal signaling, carbohydrate metabolism, cell wall, cell size, and cell cycle related biological process. To validate the expression profiles obtained by RNA-Seq, real-time qPCR was performed on 24 genes selected from key significantly enriched pathways. Comprehensive analysis suggested that paclobutrazol blocks GA signal that can effectively inhibit scape elongation; the GA signal interact with other hormonal signals including auxin, ethylene, brassinosteroid and cytokinins, and trigger downstream signaling cascades leading to metabolism, cell wall biosynthesis, cell division and the cycle decreased obviously, and finally induced dwarfism trait. Furthermore, AP2/EREBP, bHLH, C2H2, ARR, WRKY and ARF family's transcription factors were involved in the regulation of scape development inA. praecox. This transcriptome dataset will serve as an important public information platform to accelerate research on the gene expression and functional genomics ofAgapanthus.
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影响因子：
11.6
作者：
Hosoda, K;Imamura, A;Yamazaki, T
通讯作者：
Yamazaki, T
影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
9.8
作者：
Nemhauser JL;Mockler TC;Chory J
通讯作者：
Chory J
DOI：
10.1016/s0140-6736(80)91745-6
发表时间：
1980-06
期刊：
The Federal Theology of Johannes Cocceius (1603-1669)
影响因子：
--
作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
通讯作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
影响因子：
64.8
作者：
She, Ji;Han, Zhifu;Kim, Tae-Wuk;Wang, Jinjing;Cheng, Wei;Chang, Junbiao;Shi, Shuai;Wang, Jiawei;Yang, Maojun;Wang, Zhi-Yong;Chai, Jijie
通讯作者：
Chai, Jijie